高脂饮食诱导下肥胖和肥胖抵抗大鼠细胞形态及瘦素AMPK表 达变化研究.docVIP

高脂饮食诱导下肥胖和肥胖抵抗大鼠细胞形态及瘦素AMPK表 达变化研究 　　摘 要： 目的：旨在发现肥胖和肥胖抵抗大鼠体脂、细胞形态、瘦素、AMPK发生的变化，研究 肥胖抵抗发生的机制，为预防人类肥胖，调整不合理的饮食结构，降低疾病的发生提供一定 的理论依据。方法：选取3－4周龄离乳SD雄性大鼠90只，适应性喂养1周后，随机分为2组， 正常对照组（NS）9只、高脂饮食诱导组（HS）81只，通过13周的高脂饮食诱导催肥，选出 肥胖大鼠8只，肥胖抵抗大鼠8只，进行体重，体脂，细胞形态学、瘦素与AMPK活性测试。结 果：1)高脂饮食喂养导致大鼠肥胖、体脂增加，同时出现肥胖和肥胖抵抗两种现象；2)肥胖 抵抗大鼠体脂百分比显著低于肥胖组大鼠，与正常对照组大鼠相比有升高趋势；3)在相同面 积视野下，肥胖组与肥胖抵抗组大鼠肠系膜脂肪细胞数量显著低于正常对照组，而脂肪厚度 显著增加；同时肥胖抵抗组大鼠肠系膜脂肪细胞数量较肥胖组稍高，而脂肪厚度较肥胖组大 鼠下降；4)肥胖组与肥胖抵抗组大鼠瘦素水平在喂养过程中出现增加趋势且显著高于正常对 照组；5)肥胖组大鼠AMPK活性在喂养过程中出现下降趋势，肥胖抵抗组大鼠出现升高趋势且 显著高于肥胖组大鼠。结论：经高脂喂养的大鼠出现肥胖抵抗现象可能与其瘦素水平提高， 从而促进AMPK活性提高有关。 　　关键词：高脂饮食；肥胖；肥胖抵抗；瘦素；AMPK活性 　　中图分类号：G804.7文献标识码：A文章编 号：1007-3612(2011)03-0067-05 　　 　　Research on the Variance of Tissue Morphology, Leptin and AMPKbetween Obesity and 　　Obesity resistant Ratsunder High fat Diet Inducement 　　GUO Guang1，TIAN Rong2, QU Hui3, LI Jie4 　　(1.P.E. Department, Northwest Polytechnical University, Xi’ an 710072, Shaanxi China; 　　2.P.E.Department, Northwest Polytechnical Un iversity Ming De College, 710072, Shaanxi China; 　　3.School of Engineering , Chinese People’s Armed Police Force, Xi’an 710086, Shaanxi China; 　　4 .Institute of Technology, Chinese People’s Armed Police Force, Xi’an 710086,Shaanxi China) 　　Abstract: Objective：This article’s aim was to discover the variance of body w eight, body fat, tissue morphology, leptin and activity of AMPK between obesityand obesity resistant rats and research the mechanism of DIO R,provide some t heoretical basis forprevention of human obesity, the readjustment of irrationa l diet, reduce the incidence of disease.Methods: 90 male weanling SD rats aged3 4 week, adaptive feeding for one week were randomly divided int o two groups, 9 in normal control group (NS), 81 in high fat diet induced grou p (HS), through the 13 week high fat diet induced fatten to elect eight obeserats and eight obesity resistant rats to measure body weight, body fat, cell m orphology test, test leptin in blood and activity of AMPK. Results:1)Rats whi ch fed b