黑素对人表皮渗透屏障功能和超微结构影响研究.docVIP

黑素对人表皮渗透屏障功能和超微结构影响研究 　　DOI：10.16662/j.cnki.1674-0742.2017.25.007 　　[摘要] 目的 研究黑素?θ吮砥ど?透屏障功能和超微结构的影响。 方法 方便选取2014年8月―2015年8月于镇江市第四人民医院皮肤科门诊招募稳定期白癜风20例，用多功能皮肤生理仪测量黑素缺失的白癜风皮损和正常皮肤的基础经皮水分丢失及恢复率；同时，从上述患者中挑选出5例，用RuO4电子特染结合透射电镜技术比较白癜风脱色皮损和正常皮肤角质层板层脂膜超微结构。 结果 白癜风组TEWL为（11.13±1.04）g/（m2?h），对侧正常皮肤组TEWL为（10.38±1.26）g/（m2?h）；两者差异无统计学意义；白癜风TEWL的恢复速率为（41.29±4.03）%，对侧正常皮肤TEWL的恢复速率为（59.47±4.23）%，白癜风皮损恢复率低于正常皮肤。电镜下观察到正常皮肤角质层细胞间隙可见4～8层排列规整电子致密带和透明带重复交替排列板层状脂膜结构，白癜风皮损角质层细胞间隙板层脂膜结构断裂出现空泡，空泡内充填有无形电子致密物。结论 黑素缺失皮损存在存在渗透屏障功能和结构的改变，可能原因皮损由于黑素缺失，影响了板层脂膜合成相关酶类活性。 　　[关键词] 黑素；白癜风；经皮水分丢失；超微结构；板层脂膜 　　[中图分类号] R28 [文献标识码] A [文章编号] 1674-0742（2017）09（a）-0007-04 　　Effects of Melanin on the Function and Ultrastructure of Human Epidermal Osmotic Barrier 　　LIU Zi-zhong， LI Xin 　　Department of Dermatology， the Fourth Hospital of Zhenjiang， Zhenjiang， Jiangsu Province， 212001 China 　　[Abstract] Objective This paper tries to investigate the effects of melanin on function and ultrastructure of human epidermal osmotic barrier. Methods 20 outpatients with stable vitiligo recruited from August 2014 to August 2015 at Department of Dermatology in this hospital were convenient selected. The basal TEWL and the barrier recovery rate were measured on both vitiligo-involved and contralateral uninvolved sites by respective probes connected to a multifunctional skin physiological instrument. And 5 patients were selected among the above 20 patients， the ultrastructural changes of lipid lamellar membranes in the skin were assessed by a transmission electron microscopy （TEM） technique in combination with ruthenium tetroxide （RuO4） staining. Results TEWL in vitiligo-involved group was not significantly different compared with the uninvolved group[（11.13±1.04）g/（m2?h） vs （10.38±1.26）g/（m2?h）]）； at the same time， barrier recovery was significantly delayed in vitiligo-involved group compared with contralateral uninvolved group[（41.29±4.03）% vs （59.47±4.23）%]（P 　　[Key words] Melanin； Vitiligo； Transepidermal water los