超临界流体色谱提纯柠檬酸实验研究-化学工艺专业论文.docxVIP

ABSTRACT Citric acid is one of 由e main products of organic acid. It is widely applied in the field of food，pharmaceutical and cosmetics industry. The conventional separation used in the indus町 is the precipitation as a calcium salt，using calcium carbonate and sulfuric acid.ηlis conventional process includes a lot of batch steps which increase the produωion cost and generate considerable amount of an environmental harmful N was阳. Many new separation techniques，i.e.，ion exchange separation，have been developed. But the problems mentioned above were not absolutely solved. In recent 4岛 years，Supercritical fluid chromato伊叩hy (SFC) ，which is environment.仕iendlyand high-efficiency technology of separation and purification ，is widely used in refining of nature drugs and food. In this paper，SFC wωfrrst introduced into the purification of citric acid 仕om simulated fermentation. In the SFC separation for citric acid，polar column ZoxBax SB-CN (250x9.4mm I.D. ，5阳n) w;部 used as the stationary phase，and SC-C02/C2日sOH was used as the mobile phase and the content of ethanol w，础9.7% (wt.%) .ηle velocity of C02 is 15g.min.1，tempera饥lTe 313.15K，pressure 13MPa and injected volume O.05mL.Under the above conditions，the purity ofωllected citric acid reached 99.6%，removal rate of glucose reached 93% and the yield reached 87.2%. 咀lis paper focused on adso叩tion 叫uilibria of citric acid from supercritical fluid on a cyano column as stationary phase. MD method of molecular simulation w础 used to predict the density of 饵-COiC2HSOH binary mixture in the range of pressure 13MPa to 19.8MPa and at T=313.15K and T=32 1.15K. Under the conditions，the method of elution by characteristic point (巴CP) was used to determine adsorption 如. isotherm of citric acid 仕om supercritical fluid on the ZorBaxSB cyano column. The results indicated that the loading of ci仕ic acid on the stationary phase decreased with 也 increasing temperature and density of binary mobile phase. ηle Hil1