儿童幽门螺杆菌抗生素耐药性和毒力基因相关性的研究-儿科学专业论文.docxVIP

然可以使用标准的三联疗法根除幽门螺杆菌。阿莫西林的耐药率较低，可作 为根除治疗的首选药物。3.本组实验儿童幽门螺杆菌毒力基因表达以 cagA+和 vacAs1/m2 型为主。4.克拉霉素、甲硝唑和阿莫西林的耐药性与毒力基因 cagA 和 vacA 各亚型之间没有相关性。 【关键词】 幽门螺杆菌；儿童；耐药；空泡细胞毒素基因；细胞毒素相关 基因 III The relationship between Helicobacter pylori antibiotic resistance and virulence gene in children Major: Pediatrics Postgraduate: Qin Dan Supervisor: Professor Zhu Li Professor Chen Xiaoxia [Abstract] Objective: To evaluate the prevalence of clarithromycin 、amoxicillin and metronidazole resistance of Helicobacter pylori clinical isolates and the expression of virulence genes cagA and vacA in children and to investigate whether there is any relationship between drug resistance and genotype in this study.Methods: Hp strains clinical isolates were cultured from children patients(500) with upper abdominal discomfort who underwent gastroscopy during the period from January 2014 to December 2014 in Children’s Hospital of Guiyang City,rapid urease test was applied to the antrum of stomach mucosal tissue incised under gastroscope.The distribution of cagA and vacA was detected by polymerase chain reaction, and the resistance to clarithromycin 、 amoxicillin and metronidazole were detected by agar dilution methodin. Fisher exact test statistics was using to analyse the experimental data.Results:1.In 500 children with gastroscopy randomly selected,through rapid urease test to determine the helicobacter pylori infection in children with 281 cases of helicobacter pylori infection rate was 56.2% (281/500) ; 31 strains of H.pylori were isolated and cultured from gastric mucosa ;The positive of H.pylori rate was 11.03% (31/281).2.Among the 31 Hp strains clinical isolates from children,there were 6 ,5 and13strains were resistant to clarithromycinm,amoxicillin and metronidazole and the resistant rate was 19.35%(6/31),16.13%(6/31), 41.94%(13/31),respectively. No relationship was found between antibiotics and gender（P0.05),as well as different diseases(P0.05).3.The positive rate of cagA