雌激素对雌鼠慢性高眼压视网膜损伤的保护作用-眼科学专业论文.docxVIP

宁夏医科大学硕士研究生学位论文摘要 宁夏医科大学硕士研究生学位论文 摘要 II II The protective effect of estrogen on retina injury in rats caused by chronic glaucoma model Abstract Objective: To study the protective effect of estrogen on retina injury in rats caused by chronic glaucoma model, and the potential mechanism. METHODS: Fifty healthy female adult rats (Sprague Dawley) were randomly divided into group Ⅰto group Ⅴ ，each group have 10 rats. Group Ⅰwas normal rat group, treated with nothing . GroupⅡ-groupⅤ treated with ovariosteresis .On the eighth day, the group Ⅲ ~group Ⅴ received 0.2ml 17β-estrodiol 25ug/kg，50ug/kg and 100ug/kg respectively by subcutaneous injection every two days .Group Ⅱreceived vegetable oils by subcutaneous injection at same time. The group Ⅲ~group Ⅴ were induced chronic intraocular hypertension by cautenied the episcleral vein in 2 weeks after ovariosteresis . at mean time, examine variation of rats’ intraocular hypertension in different periods . Rats were executed in 2 month afte the model was been build .Then measured the consistence of estrogen in rat’s serum by RIA . Study the RGCs losing status in each group by HE . Examined the variation of expression of Bcl- 2 and bax protein by immunohistochemistry in rat retina. Results: ①Successfully established the chronic glaucoma model in rats. ②Compare with Group Ⅰ ,RGCs in group Ⅱ was obviously decreased in microscope. RGCs in Group Ⅲ、 Ⅳ and Ⅴ were lower than Group Ⅰ,but They were all higher than group Ⅱ. The trend of RGCs in Group Ⅲ、Ⅳ and Ⅴ increase had a positive correlation with consistence of hypodermic estrogen , which indicated that RGCs loss in chronic intraocular hypertension rat was decreased after inject with estrogen . ③The expression of Bcl-2 protein in group Ⅱ was down-regulation compare with other group (P0.05). The expression of Bcl-2 protein was no obvious difference between Group Ⅲ、Ⅳ 、Ⅴ and Group Ⅰ(P0.05). The III III expression of bax protein in groupⅡ-groupⅤ was increased compare with Gro