牡丹Ty1-copia类反转录转座子LTR序列的分离与其种质资源评价.pdf

Abstract GenBank, and the accession numbers are from KC519444 to KC519464. After exclusion of repeated sequences. 2. The test was based on iPBS-PCR method, amplified the objective sequence from northwest Plain varieties Red Hydrangea and Central Plains varieties Luoyanghong, after the polymorphic fragments were cloned, sequenced and analysed by DNAMAN、DNAstar and Vector NTI et al. which is the relevant bioinformatics software, 12 LTR sequences were got from peony LTR retrotransposons. The nucleotide sequence has the high heterogeneity, deletion mutation is the main behavior, the length of the nucleotide sequences varied from 313 to 894 bp, and homologous ranged from 31.1% to 65.8%. Cluster Analysis was made between the amino a MCID sequence and the listed LTR retrotransposon from different plant LTR amino a MCID sequence. It turned out that there was a high homology between some other plants. There may be a transverse transfer between the LTR retrotransposons of them. 3. Sequence-specific amplification polymorphism (SSAP) retrotransposon based molecular marker for germplasm identification and genetic relationship analysis of tree peony were investigated for the first time, and performance of each SSAP primer pair was also studied in detail. 8 retrotransposon primers were designed based on LTR sequence which isolated from Ty1-copia group retrotransposon in tree peony in test 1. After screened with 7 adapter primer Mse I or EcoR I which have 3 selective bases combinations, the test utilized 12 primer pairs