辛伐他汀对糖尿病大鼠周围神经病变影响.docVIP

辛伐他汀对糖尿病大鼠周围神经病变影响 　　摘 要目的:研究辛伐他汀对链脲佐菌素(STZ)诱导的糖尿病大鼠周围神经病变的影响。方法:50只正常SD雄性大鼠随机分为5组:①正常对照组Ⅰ(n=10);②正常对照组Ⅱ(n=10);③糖尿病模型对照组(n=10);④生理盐水治疗组(n=10);⑤辛伐他汀治疗组(STV,n=10)。各组均于疗程结束后行空腹血糖、总胆固醇、坐骨神经传导速度测定,之后取坐骨神经行免疫组化检查。结果:辛伐他汀治疗组鼠的坐骨神经传导速度(27.70±0.86 m/s)明显较生理盐水治疗组鼠(15.10±0.90m/s)及糖尿病模型对照组鼠的坐骨神经传导速度(15.94±0.85m/s)增快。各组治疗前后血糖、总胆固醇相比无明显差异(表4)。免疫组化病理切片光镜观察显示:STV治疗组鼠的CD34、nNOS、eNOS、VEGF抗体阳性表达明显较生理盐水治疗组及糖尿病模型对照组鼠增多,与正常对照组相近。结论:辛伐他汀可改善STZ大鼠糖尿病神经病变,这种作用不依赖其降脂效应,而和神经滋养血管的恢复有关,其机制与坐骨神经的nNOS、eNOS、VEGF表达上调有关。。 　　关键词 辛伐他汀;糖尿病神经病变;神经滋养血管 　　 　　AbstractObjective: To study the influence of simvastatin on peripheral neuropathy in streptozocin(STZ)-induced diabetic rats Methods: Fifty normal male SD rats were randomly divided into 5 groups: ①Normal control groupⅠ(n = 10);②Normal control groupⅡ(n = 10);③Diabetic model control group(n = 10); ④Saline treatmentgroup (n = 10); ⑤Simvastatin treatment group(ATV, n = 10).each rat of all groups were detected fasting blood glucose, total cholesterol, sciatic nerve conduction velocity after a period of treatment finished,following these immunohistochemistry inspection of the sciatic nerve were allowed .Results: Simvastatin treatmentgroup demonstrated obvious improvement in sciatic nerve conduction velocity(27.70±0.86 m/s) compared with the saline treatment group(15.10±0.90m/s) and the control group of diabetic model(15.94±0.85m/s). Blood glucose and serum total cholesterol levels before and after treatment were not significantly different among all groups(Table4). Simvastatin treatmentgroup demonstrated obvious improvement inCD34 、nNOS、eNOS、VEGFantibodyexpression in sciatic nerve compared with the saline treated group and the control group of diabetic model through the pathological biopsy,similar to normal rats. Conclusion:These data indicate that simvastatin have a favorable effect on diabetic neuropathyindependent of its cholesterol -lowering effect ,but relate to the resumption of vasa nervorum. Our data provide evidence that themechanism of this e