高蛋氨酸转基因大豆的鉴定和遗传稳定性分析-作物学;作物遗传育种专业论文.docxVIP

Classified Index： Code：10224 Confidential(yes／no)： NO．120310187 Dissertation for the Master Degree Identification and Genetic Stability Analysis of Transgenic Soybean with an Enhanced Level of Methionine C andidate：Qingmei Han Supervisor：Professor Tianfu Han Degree C ategory：Master of Agriculture C ollege：College of Agriculture First level discipline：Crop Science Second level discipline：Crop Genetics and Breeding Harbin China June 2015 万方数据 ————}一煳糖置皇曼鼍罾量量曼量量皇曼量量璺罾暑曼量量量皇量量暑曼曼量曼皇量曼置曼曼皇量量罾置暑量曼 丫7 7 7■^j==。竺’ 目录 一v‘ 万方数据  东北农业大学农学硕士学位论文 3结果与分析 ．23 3．1转AtD．CGS大豆遗传稳定性检测 ．．23 3．1．1 PCR和Southern blot检测结果 23 3．1．2目的基因在转录水平上的表达情况 ．．25 3．1．3目的基因在翻译水平上的表达情况 ．．27 3．1．4转基因株系中作CGS．ZGll的总蛋氨酸 一27 3．1．5转基因株系中作CGS．ZGll的农艺性状调查 ．．28 3．2高蛋氨酸转基因大豆新材料的鉴定 30 3．2．1除草剂抗性鉴定结果 ．30 3．2．2分子鉴定结果 ．．3 1 3．2．3新材料主要性状调查 ．3 1 3．2．4新材料的品质分析 ．31 3．3转AtD．CGS高蛋氨酸大豆的启动子分析 ．．33 z；讨论 ．．35 4．1转基因高蛋氨酸大豆的应用价值 35 4．2夕?源基因在转基因材料中稳定遗传 35 z．：转基因高蛋氨酸大豆的杂交转育 36 4．4关于后续工作的建议 ．．36 5结论 ．38 致{射 38 参考文献 ．39 攻读硕士学位期间发表的学术论文 40 11 万方数据  CONTENTS CoNTENTS Abstract in Chinese． ． ．．： ． ．．I Abstract iwEnglish ．． ． ．．．．．． ．．．． ．．． ．．．．． ．．． ．． ．．．． ．．．．． ． ．． ．．． ．．． ．．． II 1．Introduction 1 1．1 Synthesis and regulation ofmethionine in SOybean．．．．．． ．．．．．．．．．．．．．．．．．．．． ．．．．．．．．．．．．．． ．．．．．．．．．．．．．．1 1．1．1 Metabolic process ofmethionine in soybean ． ． ． ． ．1 1．1．2 The research status ofimproving contents ofthe methionine in SOybean．．．．．．．．． ．．．．．．． ．2 1．1．3 Improve the contents ofmethionine in soybean by the key enzyme CGS ．3 1。2 Expression and genetic stability of exogenous genes in transgenic plants．．．．．．．．．．．．．．．．．．．．．．．．．．．．．3 1．2．1 Significance of genetic stability analysis ． ．．． ．．． ．．，． ． ．． ． ．． ．． ．．．4 1．2．2 The main factors of effecting the stability of exogenous gene．． ．．． ．． ．．．．． ．． ．．． ．5 1．2．3 Methods of detecting genetic stability ．． ．． ．． ．．．． ．．．． ． ．． ．． ．．．． ．．． ．．． ．6 1．3 Get new soybean