发酵液中γ聚谷氨酸的分离纯化及初步鉴定研究轻工技术与工程专业论文.docxVIP

际将提取条件调整为[C4mim]C1浓度为404mg／mL、K2HP04加入量为1．59、 际将提取条件调整为[C4mim]C1浓度为404mg／mL、K2HP04加入量为1．59、 pH为9．O。对提取条件进行验证，得至Uy-PGA上相收率为96．63％，与理论 预测值相近，说明模型的可信度高。通过响应面试验还可以得到这三个因素 对T-PGA提取率影响的强弱顺序：pHK2HP04加入量[C4mim]Cl浓度。 (4)综合考虑萃取效果、成本和工业可行性，最终选择正丙醇．Na2C03 双水相体系提取发酵液中的丫_PGA。将下相溶液透析、冷冻干燥后，得到乳 白色粉末。茚三酮比色法结果表明P PGA的纯度为56．85％，纸层析结果表 明发酵得到的Y-PGA是由单一的谷氨酸组成，不含有其他游离氨基酸。 SDS．PAGE凝胶电泳结果表明1，．PGA的分子量范围在669kDa-700kDa之间， 是一种多分子量聚合物。 (5)对Y-PGA进行保水性和吸水性研究，保水性研究结果表明Y-PG}A 确实有保水性，而且浓度越大，保水性越强；吸水性研究结果表明经过紫外 线照射的丫．PGA的吸水性增强，且照射时间越长，吸水性越强。 关键词：十聚谷氨酸，双水相萃取，提取工艺，初步鉴定 万方数据 Study Study on Separation and Purification of y-Polyglutamic Acid from Fermentation Broth and Its Preliminary Identification ABSTRACT As a kind of macromolecular polymer，y-polyglutamic(y-PGA)is biocompatibility,biodegradability，non-toxicity and non-pollution．Compared with chemical synthesis， enzymatic conversion， extaction, microbial fermentation，a simple process and high yield method，has become the mian pathway for producing y-PGA．However，y-PGA broth composition is very complex and the’vicoity is overhigh．So seeking method for separation and purification of y-PGA from broth has become hot spot and difficulty． Traditional methods for separation and purification of 7-PGA include precipition method of organic solvents，chemical precipition and membrane separation．The first two methods have disadvantages of low extraction efficiency and low purity．The membrane was easy to plug and expensive．To improve these shortages，the paper intended to extract effectively y-PGA from broth by choosing suitable aqueous two-phase system(ATPS)．Extracting 7-PGA from broth with PEG—potassium phosphate ATPS，propyl alcohol-Na2C03 ATPS and[C4mim]C1一K2HP04 ATPS，the influence of process and environmental conditions on partition coefficient K and extraction efficiency Y were studied． And y-PGA extracted from fermentation broth was characterized preliminarily． The main content and resuks are as follows： The phase separation and extraction ability of different A