拮抗冬枣黑斑病菌链霉菌的获得及其产物活性研究-生物化学与分子生物学专业论文.docxVIP

II II Abstract The actinomycetes strains were screened from Zhanhua dong zao cultivation plots. An actinomycete strain had strong inhibition activity to Alternaria tenuissima was screened by agar block method. Then it was classified and identified. The study aimed at searching antagonistic actinomycetes and evaluating their potential in biocontrol of Black Spot disease in Jujube. Main contents and results of the study were listed as follows: Microbial quantity and nutrient in the soil with different character of Zhanhua dong zao cultivation plots were studied. The result indicated that the surface of root soil was markedly more favorable to microbe in the soil，which represented root domino effect. As agricultural utilization degree increased，the microbial number increased. In this area，bacteria were main members of microorganisms，followed by actinomyces，fungi. All the members of actinomyces strains were complex ones. And the streptomyces can be divided into nine florae. The nutrient in clay soil was higher than that in sandy soil，but bacteria，actinomyces，fungi and microbial quantity in sandy soil were higher than those in clay soil，because sandy soil is well ventilated and generally advantageous to the growth of microorganisms，it shows that there were many factors affecting the growth of soil microbes. 520 actinomycetes strains were isolated from Zhanhua Dong Zao cultivation plots. Eight strains of actinomycetes with antagonism were screened from the isolated actinomycetes by agar block method. An actinomycete strain had strong inhibition activity to Alternaria tenuissima by rotation-flask experiment，with an inhibitory cycle of 30 mm in diameter. The extracts from intracellular components also had inhibiting activity. K14 was proposed as Streptomyces alboflavus. We further analyzed the phenotypic and physiological characteristics and 16S rDNA sequences of strain K14，and identified this strain as the species Streptomyces alboflavus preliminarily. By means o