电针对缺血再灌注大鼠脑水肿与水通道蛋白-4的影响.pdfVIP

中 文 摘 要 目的：通过观察电针对缺血再灌注大鼠脑水肿及水通道蛋白－４的影响，探讨针 刺治疗缺血再灌注脑水肿的可能作用机理，为临床上运用针刺疗法治疗缺血再灌注脑水 肿提供一定的理论依据。 方法：采用线栓法制备大鼠大脑中动脉（MCA）缺血再灌注模型,随机分为假手术组， 模型组(缺血2h再灌注6h组，缺血2h再灌注24h组，缺血2h再灌注72h组）；针刺组（缺血 2h再灌注6h针刺组，缺血2h再灌注24h针刺组，缺血2h再灌注72h针刺组），采用干湿重法 检测缺血再灌注大鼠脑组织含水量，采用免疫组织化学方法检测缺血再灌注大鼠水通道 蛋白-4(AQP-4)的表达情况。 结果：（1）假手术组大鼠脑组织含水量呈基础水平，模型组大鼠脑组织含水量较 假手术组明显增多(Ｐ0.01)，至72h达到最高；针刺组大鼠脑组织含水量较相应各时段 模型组有所降低(P0.01或P0.05)，但仍高于假手术组(Ｐ0.01)。（2）假手术组AQP-4 在脑组织呈基础水平表达，模型组AQP-4均较假手术组表达增强(Ｐ0.01)，至72h达到最 高；针刺组大鼠AQP-4较相应各时段模型组有所降低(P0.01或P0.05)，但仍明显高于假 手术组(Ｐ0.01)。 结论：（1）脑缺血再灌注后，大鼠脑组织中AQP-4表达增强，并且与脑水肿程度呈 正相关，当缺血2h再灌注72h时，脑水肿程度最为严重，AQP-4阳性细胞数表达最高。（2） 针刺可降低缺血再灌注后AQP-4表达，减轻缺血再灌注后脑水肿的程度。说明针刺治疗缺 血性脑水肿的作用机制可能与其抑制脑组织内AQP-4表达有关。 【关键词】： 针刺；缺血再灌注；脑水肿；水通道蛋白-4； -5- Abstract Objective：To investigate the mechanism of acupuncture in cure brain edema after cerebral ischemic reperfusion(CIR) and to provide the basis of theory for the treatment of acupuncture in clinic by observing the effects of electro-acupuncture(EA) on the expression of aquaporion-4(AQP-4) and content of cerebral fluid after CIR . Methods：CIR models were established by occluding unilateral middle cerebral arteries(MCA) of rat with nylon filament.These rats were divided into false operation group,model group( blood flow blocked 2 hours and the reperfusion for 6h,24h,72h), acupuncture group(blood flow blocked 2 hours and the reperfusion for 6h,24h,72h),and observe the effects of electro-acupuncture(EA) on the expression of aquaporion-4(AQP-4) by immunohistochemical method and content of cerebral fluid after CIR by dry-wet method. Results: (1) The rat’s cerebral fluid content in sham operation group showed a basal level, The rat’s cerebral fluid content in model group was obviosly higher than that in sham operation group (Ｐ0.01),and it reached the peak at 72h after CIR