马棘抗炎化学成分的分析-中药学专业毕业论文.docxVIP

华中科技大学硕士学位论文 华 中 科 技 大 学 硕 士 学 位 论 文 4 4 RAW264.7 macrophages compared with the control group, the difference was statistically significant (P 0.05). The second:studies on chemical constitutes Eighteen Compounds were isolated and separated from the Petroleum ether and EtOAc extracts of the stems and leaves of Indigofera pseudotinctoria Mats by using solvent extraction, silica gel column chromatography, Gel column chromatography(LH-20), MCI column chromatography and Re-crystallization, and eighteen known compounds were identified by various modem spectral analysis (1H- NMR,13C-NMR, HSQC, H-HCOSY, HMBC) and classical chemical methods. Their structures were elucidated as: hentriacontanol(IP.1), 3β-Acetoxy-12-oleanen-11- one(IP.2), 3β-Hydroxyglutin-5-ene (IP.3) , heptacosane acid (IP.4) , Lup-20(29)-en-3- one (IP.5), β-sitosterol (IP.6) , Friedelin(IP.7) , Afromosin(IP.8), genistin (IP.9), 5,7,4‘-Hrihydroxy-3’-methoxyflavone (IP.10), sulfuretin (IP.11), 8-Hydroxy-5- indolizidinone (IP.12), butein (IP.13), 2’,4’,4- Trihydroxy chalcone(IP.14), Heptadecanoic acid-2,3-dihydroxypropyl ester(IP.15) , 2’,7-Dihydroxy-4,5- dimethoxyisoflavone(IP.16) , 4’,7-Dihydroxy-3-methoxy- isoflavone (IP.17), calycosin(IP.18). All these compounds were isolated from this plant for the first time , especially compound IP.12 was isolated from nature plant for the first time. The third: the studies on activity of four compounds in vitro We study the effects of the compounds IP.2, IP.3, IP.5 and IP.8 on growth of value-added in lipopolysaccharide (LPS)-induced mouse RAW264.7 macrophage and expression of NO in the lipopolysaccharide(LPS)-induced RAW264.7 mouse. The results show that: compounds IP.2, IP.3, IP.5 on lipopolysaccharide (LPS)-induced mouse RAW264.7 macrophages inhibited the expression of NO differently. Compared with the control group, the difference was statistically significant (P 0.05). KEY WODRS: Indigofera pseudotinctoria Mats, chemical constituent, triterpene,