沙田柚花粉壁蛋白和酸柚花粉壁蛋白的比较-生态学专业论文.docxVIP

广西师范大学硕士研究生学位论文 广西师范大学硕士研究生学位论文 PAGE PAGE 3 The Comparision of Protein from Pollen Wall Between Citrus grandis Var.shatinyu Hort. and Citrus grandis Abstract Pollen of Citrus grandis var.shatinyu Hort. and Citrus grandis are chosen as experimental material. The protein abstract solution from pollen wall of shatinyu and Citrus grandis, which is abstracted in different time stages (5min.10min.20min.30min.40min.50min.60min.3h) by pH6.7 1% sodium chloride solution which contains 10% saccharose, 0.45mM Ca2+(Cacl2 ·6H2O), 1.63 mM B(H3BO3) in icebath, is dialyzed. And then the optical density of them is tested respectively by the ultraviolet spectrophotometer at 280nm spectra. As far as the method of extraction is concerned, it is an improved one just because it speeds up the experiment process. The result shows as that follows: (1) The protein content of abstract solution from pollen wall of shatinyu and Citrus grandis in 40min. increased with time. It reached its peak in 40 min and from that point on, it slowed down and maintained a certain level, as for shatinyu, of 0.364 mg/ml; Citrus grandis, 0.488 mg/ml. （2）The protein content of abstract solution from pollen wall of shatinyu and Citrus grandis in 5min. came to half of the total amount, which coincides with the study on Oenothera organensis by Lewis.（3）We make a further study on the releasing behavior of the protein from Pollen Wall of shatinyu and Citrus grandis by microsoft，and discovered that the latter is larger in quantity and quicker in speed. Moreover, we compared the protein abstract solution from pollen wall of shatinyu with that of Citrus grandis by the means of electrophoresis such as PAGE、SDS、IEF、IEF/SDS. The result showed as follows: (1) By the means of PAGE in combination with Coomassie brilliant blue R-250 dyeing, the protein abstract solution from pollen wall of both shatinyu and Citrus grandis shows 7 obvious segments. Moreover there is a brand named A in segmentⅤin both of their PAGE coll