理筋手法对外周炎性痛大鼠局部镇痛作用及其机制的实验研究.docVIP

理筋手法对外周炎性痛大鼠局部镇痛作用及其机制的 实验研究 【摘 要】目的：探讨理筋手法对炎症性疼痛大鼠的局部镇痛作用及 其机制。方法：将24只SPF级健康雌性SD大鼠随机分为空白组、模型组、 手法组，每组8只。除空白组外，其他2组在大鼠足底皮下注射0. 1 mL 弗氏完全佐剂，建立佐剂性外周炎性痛模型，通过在疼痛局部施以理筋手 法，观察手法对大鼠炎症反应、疼痛阈值及局部腺甘含量的影响。结果： 造模后，大鼠出现致炎侧足跛行、舔爪、红、肿及热痛敏等反应。造模局 部组织可见炎性细胞浸润，肌纤维的萎缩等。手法能抑制弗氏佐剂导致的 大鼠缩足、舔爪等自发痛行为，提高炎性痛大鼠的疼痛阈值(P 0. 05)； 且能提高大鼠足底病灶局部腺昔含量，手法组局部腺背含量与空白组、模 型组比较，差异有统计学意义(P 0. 05)o结论：理筋手法局部镇痛效 果显著，其局部镇痛作用可能通过局部腺昔释放而起作用。 【关键词】理筋手法；完全弗氏佐剂；炎性痛；镇痛作用；腺昔； 大鼠 Experimental Study on the Local Analgesic Effect of Tendon-Soothing Manipulation on Rats with Periphery InflamniQtory Pain and Its Mechanism ZHAOLi-jing, LI Pei, ZHANG Zhong-bo, CHEN Yan-wei, SHI Peng-bo, BA Zheng [ABSTRACT】0bjective： To investigate the local analgesic effect of tendon-soothing manipulation on rats with periphery inflammatory pain and its mechanism?Methods： Twenty four healthy female SD rats were randomly divided into a blank group, a model group and a manipulation group, with 8 rats in each group? Except the blank group, the other two groups were given subcutaneous injection of 0. 1 mL FreuncT s adjuvant in the plantar to establish models with adjuvant peripheral inflammatory pain. Tend on-sooth ing manipulati on was used to observe its effect on their inflammatory reaction, pain threshold and local content of adenosine?ResuIts： After modeling, rats had the following reactions： claudication on the inflammatory side, 1 icking claws, red, swol 1 en and heat hyperalgesia. Tnflammatory cel 1 infiltration and atrophy of muscle fibers were seen in the local tissue for model ing. Manipulation could inhibit Freunds adjuvant caused spontaneous pain behaviors such as curling up legs and licking claws and lift their pain threshold (P 0. 05)? It also could improve the content of adenosine in rat s plantar lesions. The difference of the content of adenosine between the manipulation group, the blank group and the model group was statistically significant (P 〈 0. 05)? Conclusion： The tendon-soothing manipulation has an obvious local analg