核医学：小动物PETCT 应用 - 第2节.pptxVIP

分子核医学的主要研究内容;报告基因显像;报告基因系统;NIS是一种膜蛋白，含13个跨膜的结构域，一个细胞外氨基末端和一个细胞内羧基末端；NIS蛋白有3个可能的N-糖基化位点；1个位于细胞外第四环，2个位于细胞外最后一环[;碘的主动转运是通过甲状腺细胞膜上的钠碘同向转运体(NIS)介导的，协同转运2个Na+和1个I－，其能量由跨膜的Na+ 梯度提供。I－通过顶膜的离子通道进入滤泡腔内，在此经过甲状腺过氧化物酶(TPO)的氧化催化，进行甲状腺激素合成;分子显像无创监测MLC-2v为启动子的双基因重组腺病毒靶向治疗心肌缺血的实验研究;;Human NIS gene for tumor radionuclide therapy and report gene imaging;Retinoic acid and tributyrin restore hNIS gene expression and radioiodine uptake in a poorly differentiated follicular thyroid carcinoma. Baculovirus-mediated transfer of hNIS gene into tumor cell for radionuclide therapy and imaging. A novel positive feedback effect of 131I-promoted Bac-Egr1-hNIS expression in malignant glioma via baculovirus. Baculovirus-mediated transfer of hNIS gene as an ideal radionuclide reporter gene imaging for monitoring the fate of human stem cells.; All-trans retinoic acid (ATRA) or/and tributyrin (TB) induces radioiodine uptake in FTC-133 cells. ; Preclinical tumor therapy research. Tumor control rate: 41.2% Promising strategy for clinical tumor gene therapy.;Dose-response transfect efficiency of 131I-stimulated hNIS expression with Bac-Egr1-hNIS. ; The use of MOI= 800 in hUCB-MSCs yielded the highest GFP+ %. GFP expression of infected hUCB-MSCs was especially stable in the first 4 days and remained at a high level for at least 1 week after infection. ;1, Bac-NIS-infected hUCB-MSCs; 2, mock-infected hUCB-MSCs; 3, thyroid; 4, heart; 5, stomach; 6, intestinal area; 7, bladder.;Human plasminogen kringle 5 for anti-angiogenesis therapy;Preparing and radionuclide labelled recombinant human plasminogen kringle5 (rhk5) Baculovirus Mediated Experimental Research on Targeted Egr1-Kringle 5 Gene Radiotherapy in Lung Adenocarcinoma Baculovirus Vector-Mediated Transfer of Sodium Iodide Symporter and Plasminogen Kringle 5 Genes for Tumor Radioiodide Therapy ;;99mTc-rhk5;;Scheme of Radio-inducible K5 gene expression. As Egr1 promoter is activated by 131I and may promote K5 expression.;SPECT images at 0.5