贡菊花与野菊花薄层色谱鉴别方法的研究.docVIP

贡菊花与野菊花薄层色谱鉴别方法的研究 摘 要 [目的]对贡菊花和野菊花进行对比鉴别，找到合适的薄层鉴别方法，从化学成分上对贡菊花和野菊花进行鉴别。 [方法]按有关文献方法及改进法分别对贡菊花和野菊花中绿原酸及蒙花苷进行薄层色谱鉴别试验。 [结果]本文中贡菊花和野菊花中绿原酸鉴别方法二和蒙花苷鉴别方法二操作简便、色谱斑点显色清晰、分离效果好。贡菊花和野菊花供试品色谱中，在与绿原酸对照品色谱相应的位置均有相同颜色的荧光斑点，贡菊花的斑点略暗于野菊花的斑点；在与蒙花苷相应的位置亦均有颜色相同的荧光斑点，野菊花的斑点明显亮于贡菊的斑点。 [结论]比较贡菊和野菊花的实验结果，贡菊花和野菊花中绿原酸鉴别方法二和蒙花苷鉴别方法二具有良好的专属性，重现性,更具有可行性，可用于贡菊花和野菊花的薄层色谱鉴别。从色谱图直观分析，贡菊花和野菊花中均含有绿原酸和蒙花苷，但其含量存在差异，本研究内容为贡菊花和野菊花的薄层色谱指纹图谱分析奠定了基础。 关键词：贡菊花；野菊花； 绿原酸； 蒙花苷； 薄层色谱法 Study on identification of the tribute chrysanthemum and wild chrysanthemum of thin-layer chromatography Abstract [Purpose]:Compared to identify the tribute chrysanthemum and wild chrysanthemum ,and find the right TLC method to identify them from the chemical composition. [Method]: Making a TLC identification test on the chlorogenic acid and Linarin in the tribute chrysanthemum and wild chrysanthemum according to the literature method and improvement method. [Outcome]: The second method to identify chlorogenic acid with Linarin in the tribute chrysanthemum and wild chrysanthemum is easy to operate, and chromatographic spots is clear, and separation is good, the tribute chrysanthemums and wild chrysanthemums test products for chromatography, The corresponding position in the chlorogenic acid reference standard chromatographic have the same color fluorescent spots, The tribute chrysanthemums spots slightly darker than wild chrysanthemums spots;The corresponding position in the Linarin reference standard chromatographic have the same color fluorescent spots, The wild chrysanthemums spot is noticeably brighter than the tribute chrysanthemums spots. [Conclusion]: Compare the experimental results of the tribute chrysanthemum and wild chrysanthemum, The second method to identify chlorogenic acid with Linarin in the tribute chrysanthemum and wild chrysanthemum is good on specificity, reproducibility which can be used to make a TLC to identify The tribute chrysanthemum with wild chrysanthemum . Visua