葡萄糖神经酰胺合酶与白血病细胞耐药的关系.docVIP

葡萄糖神经酰胺合酶与白血病细胞耐药的关系 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：葡萄糖神经酰胺合酶与白血病细胞耐药的关系 1 文2：醛固酮合酶基因多态性与老年原发性高血压的关系 6 1 对象与方法 6 2 结果 8 3 讨 论 9 参考文摘引言： 10 原创性声明（模板） 11 文章致谢（模板） 12 正文 葡萄糖神经酰胺合酶与白血病细胞耐药的关系 文1：葡萄糖神经酰胺合酶与白血病细胞耐药的关系 Relation Between Glucosylceramide Synthase and Multidrug Resistance in Leukemia Cells AbstractThis study was purposed to explore the expression of glucosylceramide synthase (GCS) in human leukemia cells and its relatiohip with multidrug resistance. RT-PCR was used to analyze peripheral blood samples from 53 leukemia patients with multidrug resistanceon-resistance, and to detect the expression level of GCS gene in HL-60 cells and HL-60/ADR cells, the expression level was compared with the level of mdr-1. The expressio of GCS protein and P-gp prolein in HL-60 cells and HL-60/ADR cells were assayed by Western blot analysis. The results showed that the relative optical deity ratio of GCS gene amplified bands in samples of leukemia patients with drug- resistance was significantly higher than that in samples of leukemia patients with drug non-resistance group (P), meanwhile the significant enhancement of optical deity value of GCS gene amplified bands accompamied by high expression of mdr-1 gene. Their correlation showed positive (P, r=). The GCS mRNA and protein were overexpressed in HL-60/ADR cells, and their expression levels were obviously higher than that in HL-60 cells, meanwhile the expression of mdr-1 mRNA and P-gp also significanfly increased in HL-60/ADR cells. It is concluded that the high level of GCS in leukemia patients possibly is associated with multidrug resistance of leukemia cells. Key wordsglucosylceramide systhase; multidrug resistance; leukemia; RT-PCR; Western blot analysis 化疗是 治疗 白血病的主要手段，而多药耐药（multidrug resistance, MDR）导致的化疗失败则是白血病治疗中困扰临床的重大难题。目前国内对MDR的研究较集中在药物转运泵的作用致药物外排增加从而产生耐药。为了进一步探讨白血病耐药的相关机制及寻找逆转白血病细胞耐药的靶标，我们将从另一条新的途径即神经酰胺信号系统中葡萄糖神经酰胺合酶（glucosylceramide synthase, GCS）的表达水平对白血病细胞耐药作用进行研究。 材料和方法 临床标本 5