重组腺病毒对HelaMCF7的转染效率及其介导的hTRAIL基因体外抑瘤作用临床医学.docVIP

重组腺病毒对HelaMCF7的转染效率及其介导的hTRAIL基因体外抑瘤作用临床医学 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：重组腺病毒对HelaMCF7的转染效率及其介导的hTRAIL基因体外抑瘤作用临床医学 1 1 材料与方法 3 2 结 果 4 3 讨 论 5 文2：腺病毒介导血管内皮细胞生长因子基因体外转染骨髓间充质干细胞 6 1 材料和方法 7 2 结 果 10 3 讨 论 12 参考文摘引言： 13 原创性声明（模板） 15 文章致谢（模板） 15 正文 重组腺病毒对HelaMCF7的转染效率及其介导的hTRAIL基因体外抑瘤作用临床医学 文1：重组腺病毒对HelaMCF7的转染效率及其介导的hTRAIL基因体外抑瘤作用临床医学 Study on the trafection efficiency of recombinant adenovirus to Hela cell and MCF7, and preliminary observation on the in vitro antitumor effect of hTRAIL gene mediated by adenovirus 【Abstract】 Objective To study the infection efficiency of recombinant adenovirus to uterine cervix cancer cell and MCF7, and observe the effect on the growth of uterine cervix cancer cell Hela and MCF7 of hTRAIL gene mediated by recombinant adenovirus. Methods After Hela cells and MCF7 cells were added to wells of 12well plates and incubated for 24 h, the different MOI of AdCMVEGFP virus were added into the 12wells, infected by recombinant adenovirus, infection efficiency was detected by flow cytometry. The adenovirus(AdCMVhTRAIL) were trafected after Hela cells and MCF7 cells being added to the 96well plates for 24 h, and the survival rates were detected using MTT method 72 h later.　Results Within a certain range, the infection efficiency of recombinant adenovirus to uterine cervix cancer cell and MCF7 increased with increasing of MOI. The survival rates of Hela cell trafected with AdCMVhTRAIL were significant lower than the control group trafected with AdCMVEGFP. Conclusio By recombinant adenovirus to infect uterine cervix cancer cell and MCF7 can increase the infection efficiency, and hTRAIL gene mediated by adenovirus has antitumor effect on Hela in vitro. 【Key words】 Hela; MCF7; Tumor necrosis factorrelated apoptosis inducing ligand(TRAIL); Recombinant replicateddeficient adenovirus; Gene therapy 肿瘤坏死因子(TNF)相关的凋亡诱导配体(TNF related apoptosis inducing ligand，TRAIL)是TNF家族成员之一。目前研究表明，TRAIL