成团泛菌的研究现状.pptVIP

成团泛菌的研究现状 呼吸科 林琦 同一病区同一阶段感染成团泛菌两例 病例一 男，82岁，脑梗塞长期卧床，尿培养为成团泛菌。 病例二 女，82岁，脑梗塞长期卧床，突发寒战高热，两次血培养均为成团泛菌。 The growth conditions of Pantoea agglomerans, a phosphate solubilizing organism, were studied in our laboratory to determine the optimal conditions. Pantoea agglomerans showed the highest growth rate at 30°C, pH 7.0 and 2 vvm, after 50 h cultivation. A certain relationship between pH and phosphate concentration was evident when the glucose concentration in the medium was changed. Increasing glucose concentration increased the pH buffer action of the broth.At glucose concentrations higher than the optimum concentration of 0.2 M, the cell growth was retarded. P. agglomerans consumed glucose as a substrate to produce organic acids which caused the pH decrease in the culture medium. The phosphate concentration in the medium was increased by the presence of the organic acids, which solubilized insoluble phosphates such as hydroxyapatite. A 52-year-oldman undergoing continuous ambulatory peritoneal dialysis presented with two consecutive episodes of peritonitis caused by unusual organisms , namely, Brevibacterium and Pantoea agglomerans. The patient was successfully treated with a 2-week course of cefazolin and ceftazidime for the Brevibacterium-associatedperitonitis, and a3-week course of gentamicin for the P. agglomerans-associated peritonitis. Although these environmental organisms are rarely responsible for human infection, the number of reported cases of human infection by these unusual organisms has increased.This report emphasizes the potential for infection by environmental organisms in patients undergoing peritoneal dialysis. Pantoea agglomerans BH18, isolated from mangrove sludge, could produce hydrogen under marine culture condition. To improve the hydrogen-producing capacity of this strain, we constructed a stable transposon-mutagenized library of P. agglomerans BH18. A Tn7-based trans