锌镧促进香稻糙米香气2-乙酰-1-吡咯啉形成机理的研究.pdfVIP

锌镧促进香稻糙米香气2-乙酰-1-吡咯啉形成机理的研究 唐湘如，肖迪，黄锦霞，黎国喜，潘圣刚，段美洋，田华，钟克友，肖立中 （华南农业大学农学院，广州 510642） 摘要：以香稻香味等近基因系等为材料，进行了基肥施用Zn、La肥和 Zn、La、脯氨酸（Pro）和2-乙酰-1- 吡咯啉（2-AP）离体穗培试验。结果表明：香稻糙米中2-AP含量最高的Zn和La基肥用量为ZnCl2 80-100mg/kg 土壤和LaCl3 100mg/kg土壤；基肥Zn和La 肥能显著增加香稻糙米2-AP 含量、伤流液中的Zn、La 和 2-AP 含量 ；适当增加穗培Zn浓度显著提高香稻籽粒的Zn含量、脯氨酸氧化酶(PO)和吡咯啉乙酰基转移酶(PA) 活性、2-AP 含量；穗培 La 浓度的增加促进香稻籽粒 pro 含量、PO、PA 活性和 2-AP 含量的提高,但对香稻 籽粒La含量影响不大；适当增加穗培pro浓度能促进香稻糙米中Pro含量、PO和PA活性、2-AP合成积累； 穗培直接供应香稻籽粒2-AP 能显著促进其糙米2-AP积累；调控香稻糙米2-AP形成积累主要的生理生化性 状为茎2-AP含量、剑叶pro 含量、根pro 含量和籽粒PO 活性；调节糙米香气2-AP合成积累的最重要合成 酶为 PO。探明了锌镧促进香稻糙米香气 2-AP 形成的生理生化机制。对浓香香稻品种选育及其高香栽培具 有重要的理论和实践意义。 关键词：香稻;2-乙酰-1-吡咯啉;锌;镧;形成机理 Mechanism of zinc and lanthanum promoting formation of 2-acetyl-1-pyrroline in aromatic brown rice Tang Xiangru, Huang Jinxia, Xiao Di, Li Guoxi, Pan Shenggang, Duan Meiyang, Tian Hua, Zhong Keyo, Xiao lizhong （College of Agriculture, South China Agricultural University, Guangzhou 510642, China ） Abstracts:The experiments of basic application of zinc and lanthanum fertilizers and of zinc, lanthanum, proline and 2-acetyl-1-pyrroline(2-AP) in panicles culture were carried out to mechanism of zinc and lanthanum promoting formation of 2-acetyl-1-pyrroline in aromatic brown rice by using near-isogenic aroma rice lines. The main results showed that: the contents of 2-AP in aromatic brown rice got their maximums at basic using 80-100mg ZnCl2 , 100mg LaCl3 per kilo soil. Zn and La basic application significantly increased 2-AP content in aromatic brown rice, zinc, lanthanum and 2-AP content in xylem sap. Reasonable concentration of zinc in panicle culture significantly increased zinc content, activity of proline oxidase (PO) and pyrroline-ace