环境胁迫对栉孔扇贝（Chlamys farreri）血浆蛋白及其抗菌活性的影响.pdfVIP

第 31 卷 第 1 期 广东海洋大学学报 Vol.31 No.1 2011 年 2 月 Journal of Guangdong Ocean University Feb. 2011 环境胁迫对栉孔扇贝（Chlamys farreri）血浆蛋白及其 抗菌活性的影响 1 2 2 张维柱 ，余招龙 ，艾伟岭 （1. 滁州学院化学与生命科学系，安徽 滁州 390000 ； 2. 电白冠利达海洋生物科技养殖有限公司，广东 电白 525400 ） 摘 要 ：采集采自山东某养殖场的栉孔扇贝（Chlamys farreri ）健康贝和病贝的血淋巴，测定其血浆蛋白浓度、 血浆蛋白图谱和相对分子质量，以及血浆抗菌活性、凝集素活性等几种重要的体液免疫因素，研究环境胁迫对 血浆蛋白及其抗菌活性的影响。结果表明：栉孔扇贝（C.farreri ）血浆蛋白质量浓度约 1 260 ~ 1 650 μg/mL；血 浆蛋白 SDS 指数 BI 为 0.34，病贝 BI 为 0.21；凝集素活力水平为 1.602 ~ 2.204。氨氮胁迫下血浆蛋白浓度下降约 780 μg/mL， 凝集素活力下降为 1.301；高温胁迫下血浆蛋白质量浓度下降约为 900 μg/mL，凝集素活力为 1.903；高盐胁迫下 血浆蛋白浓度下降约为 990 μg/mL，凝集素活力为 2.204。 关键词：栉孔扇贝；血浆蛋白浓度；抗菌活性；凝集活性；SDS 中图分类号：S944.4+3 文献标志码：A 文章编号：1673-9159 （2011 ）01-0062-05 Effects of Environmental Stresses on Plasma Protein and Bactericidal Activities of Chlamys farreri 1 2 2 ZHANG Wei-zhu , YU Zhao-long , AI Wei-ling （1. Department of Chemistry and Biology, Chuzhou University, Chuzhou 239000, China; 2. Dianbai Marine Biotechnology Aquaculture Co ., Ltd .，Dianbai 525400, China ） Abstract: Samples of haemolimph were taken from healthy and diseased scallops （Chlamys farreri） cultivated on a fish farm in Shandong. Several important humoral immune factors ，such as concentration of plasma protein, plasma protein profile，plasma protein molecular weight, plasma anti-bacterial activity, and lectin activity were measured ；and the effects of environmental stress on plasma protein and antibacterial activity were studied. The measurements showed that plasma protein concentration was about 1 260 - 1 650 μg/mL ；SDS relative molecular weights were 34 00, 25 000 and 14 000, respectively. Plasma bactericidal activity titers were about 0.34 in healthy plasma and 0.21 in diseased plasma. Agglutinin activities were about 1.602 - 2.204. The results of studies in laboratory showed that plasma protein concentration stressed by ammonia nitrogen was about 780 μg/mL, agglutinin activity about 1.301; and plasma protein concentratio