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GenlociBiotechnologiesInc.
GenlociBiotechnologiesInc.
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Email: sales@
ProductInformation
IntegrationOperatingSystem(IOS)
GeneTerminatorKit
Tableofcontents
ProductDescription
KitComponents
StorageandStability
Protocols
Reagentspreparation
Transfection
a.Electroporation
b.Lipid-basedtransfection
c.FuGENE®6Transfection
Resultsoftransfection
Screeningpositivecloningbyantibiotics
PrecautionsandDisclaimers
ProductDescription
ProductDescription
PPrroodduuccttDDeessccrriippttiioonn
Integration Operating System (IOS) is a cutting-edge DNA recombination
technology, which can be used for editing any target gene accurately and effectively.
Now thousands of kits for gene suppression are available for studies of gene function.
By using sequence-specific oligonucleotides invading DNA efficiently, DNA
conformational change may be induced in the target region, and can be recognized by
a specific recombinant protein and also break the DNA, which activates innate DNA
repair mechanisms to induce DNA recombination and terminate transcription
completely. Through anchoring the donor DNAat the broken DNA siteby the protein,
the accuracy and efficiency of recombination are 1000 times better than those
conventional techniques. Low efficiency and off-target occurred in DNA
recombination are solved successfully, resulting in a more convenient targeted gene
editing.
KitComponents
KitComponents
KKiittCCoommppoonneennttss
Component CatalogNumber Quantity
IOSReagent 1 10μl
IOSReagent 2 10μl
IOSReagent 3 IOS-01 4μl(1μg/μl)
IOSR
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