1,25 hydroxylation of vitamin D3 on human high-metastatic giant cell lung cancer PGCL3 cell invasion migration and adhesion ability of.docVIP

 PAGE \* MERGEFORMAT 13 1,25 hydroxylation of vitamin D3 on human high-metastatic giant cell lung cancer PGCL3 cell invasion migration and adhesion ability of 【Abstract】 Objective To investigate the 1,25-hydroxylation of vitamin D3 (calcitriol) on human high-metastatic giant cell lung cancer PGCL3 cell adhesion, invasion and migration capacity. Methods MTT assay of its PGCL3 cell growth; were associated with the adhesion of the basement membrane components analysis experiments, reconstituted basement membrane invasion assay, Transwell chemotaxis chamber movement model calcitriol PGCL3 cell adhesion, invasion and sports capacity. Results PGCL3 calcitriol significantly inhibited cell growth. Calcitriol effect PGCL3 cell 24h of the IC50 value of 0.158 μmol / L. Calcitriol 0.025,0.05,0.075 μmol / L treatment PGCL3 cells 24h, right PGCL3 cell invasion inhibition rates were 12.94%, 27.33%, 38.22%, to PGCL3 ability to inhibit cell motility rates were 8.70%, 29.83 %, 43.62%. Reduce PGCL3 cells to laminin, fibronectin adhesion rate, and its results showed a definite dose-dependent relationship. Conclusion calcitriol inhibit human high-metastatic giant cell lung cancer PGCL3 cell adhesion, invasion and migration; PGCL3 calcitriol on cell adhesion and invasion of cells with reduced PGCL3 laminin, fibronectin adhesion the rate of about. Keywords: 1,25-hydroxylation of vitamin D3; adhesion; invasion; migration; human high-metastatic giant cell lung cancer cells PGCL3 【Abstract】 Objective This study was designed to investigate the effects of 1,25-dihydroxyvitamin D3 on adhesion, invasion and migration of PGCL3 cells.Methods Cell grow inhibition was measured by MTT assay. The effects of 1,25 (OH) 2vit D3 on adhesion, invasion and migration of PGCL3 cells were determined using analysis of adhesion-related basement membrane components fibronectin and laminin, reconstituted basement membrane invasion assay, and Transwell model, respectively.Results