Huang Jing decoction 5Determination of hydroxymethylfurfural.docVIP

 PAGE \* MERGEFORMAT 10 Huang Jing decoction 5Determination of hydroxymethylfurfural Of: Wenyuan Zhen, Malan, Huangsu Fang, Xiaojun, Huang Yue-chun [Abstract] [Objective] Huang Jing decoction of 11 batches of 5-HMF content of the study, as the material basis of Polygonatum basis for the study and quality control. [Methods] obtained by boiling the test solution, efficient solution chromatography (HPLC) method for the determination of its content. Chromatographic conditions: Hypersil ODS2 column (250 mm 4 6 mm, 5 m), methanol - Score 0 05% phosphoric acid solution (volume ratio 10:90) as mobile phase, flow rate 1 0 mL / min, detection wavelength was 280 nm. [results] 11 Polygonatum decoction approved 5-HMF content in the difference of 30 times in the 0 020 0 ~ 2 000 0 g ( r = 0 999 7) a good linear relationship, the average recovery was 100 3% (sR = 1 9%, N = 6). [Conclusion] The method is simple, accurate and material basis for the research and Polygonatum provides a reference for quality control. [Keywords:] Huang Jing / chemistry; 5-HMF / analysis; high pressure liquid Huang Jing (Polygonatum sibiricum Red.) Or more of Polygonatum (Polygonatum cyrtonema Hua) rhizome dry with qi and yin, spleen, lungs, kidney function, for the treatment of spleen and stomach, body tired fatigue, dry mouth and eat less, lung deficiency dry cough, fine blood, heat Diabetes embolism [1]. Polygonatum contain more sugar composition, in the processing and storage occurs during the thermal degradation of sugar to produce 5-hydroxymethyl furfural. the traditional view that 5 HMF toxic effects on the body, is the toxic ingredients of drugs, eye, mucous membrane, skin irritation, excessive consumption can cause poisoning, resulting in human skeletal muscle paralysis and visceral damage [2]. But in recent years, their biological propose a new view of activity, research has shown that 5-HMF has obvious anti-oxidation and improve the cardiovascular system [3], can enhan