Human placental tissue source of hematopoietic stem - progenitor cells in a preliminary study.docVIP

 PAGE \* MERGEFORMAT 21 Human placental tissue source of hematopoietic stem / progenitor cells in a preliminary study Authors: Liu YF, Zhang Yongzhuo, Zhang Chuan-Xin, Wang Diao Abstract In order to explore the source of full-term placental tissue mononuclear cells (human mature placenta tissue-derived mononuclear cells, hPTMNC) in CD34 cells in vitro proliferation and differentiation capabilities, to find new hematopoietic stem / progenitor cell source for clinical applications, respectively by flow cytometry, hematopoietic stem / progenitor cells in vitro colony culture and long-term culture-free method of cytokines were measured in the placenta and umbilical cord blood source of CD34 cells and their subsets and the number of colony-forming units. The results showed that: placental tissue source of CD34 cells (2.74 ± 0.61%) and its subsets in CD34 / CD38-cells (2.46 ± 0.42%), hematopoietic stem / progenitor cell colony CFU-GM (186.90 ± 24.52) and BFU-E (101.40 ± 13.35) level higher than the umbilical cord blood CD34 cells (1.73 ± 0  32%), CD34 / CD38-cells (0.80 ± 0.25%), CFU-GM (136.90 ± 25.15), BFU-E (49.20 ± 8.13) is high; The former factor in vitro cell-free culture conditions to survive a long time, and the number of cells increased by about 2-fold. Conclusion: The placenta of the blood-forming organs, the placenta is more naive cell components, is a hematopoietic stem / progenitor cell transplantation in the new seed cells. Keywords: placental tissue Preliminary Study on Human Mature Placenta Tissue-derived Hematopoietic Stem / Progenitor Cells Abstract Clinical transplantation has indicated that cord blood (CB) can be used in the hematopoietic reconstitution in the children, but not well used in the adult patients because of the low cell amount. The present study aimed to explore the capability of proliferation and differentiation of the hematopoietic stem / progenitor cells derived from human mature placenta