Of nitric oxide in morphine treatment on myocardial ischemia-reperfusion injury in the role of.doc

 PAGE \* MERGEFORMAT 17 Of nitric oxide in morphine treatment on myocardial ischemia-reperfusion injury in the role of Of: Wang, Chao Huei Chuan, Jin Dongmei, car Yue Juan, Yu-Juan Li, Wang Fei, Peng Ling books [Abstract] [Objective] morphine on acute myocardial ischemia / reperfusion injury as well as signaling pathways eNOS / NO effect. [Methods] SD rats were randomly divided into 4 groups 56, 14 rats in each group: S group (sham surgery, wear line, and not ligation), I / R group (ischemia reperfusion), M group (morphine treatment + ischemia-reperfusion), L + M group (L-NAME + morphine treatment + ischemia-reperfusion). In addition to S group, all have experienced cardiac ischemia and 45 min 120 min of reperfusion. hemodynamic indexes during 120 min of reperfusion in each group randomly selected nine rats with TTC staining, and myocardial ischemic infarction (IA / AR); the remaining 5 rats, take heart, with the Western Blot technique of total eNOS and phosphorylated eNOS protein expression, nitrate reductase method of myocardial NO content . using SPSS 12.0 statistical software, statistical analysis using analysis of variance and SNK test. [results] and I / R group, M group significantly reduced infarct size, (32 + - 2.8)% vs (49 + - 2.9)% ( P lt;0.01); phosphorylation of eNOS protein expression was significantly increased; NO content in myocardium was significantly increased. non-selective nitric oxide synthase inhibitor, l-NAME completely eliminated after morphine treatment on myocardial protection, significantly reduced After the treatment of morphine induced myocardial tissue NO content increased, but did not inhibit the morphine treatment induced phosphorylation of eNOS protein expression increased. [Conclusion] in rat model of myocardial ischemia, post-treatment of morphine can eNOS / NO pathway, myocardial ischemia / reperfusion injury. [Keywords:] myocardial reperfusion injury; post-processing; morphine; nitric oxide Abstract: [Objective]