Bioactive Constituents of Brazilian Red Propolis英文文献资料.docVIP

AdvanceAccessPublication3May2006 eCAM2006;3(2)249–254 doi:10.1093/ecam/nel006 OriginalArticle BioactiveConstituentsofBrazilianRedPropolis BoryanaTrusheva1,MilenaPopova1,VassyaBankova1,SvetlanaSimova1,MariaCristina Marcucci2,PatriciaLagunaMiorin2,FlaviadaRochaPasin2andIvaTsvetkova3 1InstituteofOrganicChemistrywithCentreofPhytochemistry,BulgarianAcademyofSciences,1113Sofia,Bulgaria, 2FaculdadedeFarmacia,UniversidadeBandeirantedeSaoPaulo,RuaMariaCandida,1813,VilaGuilherme, CampusMC,02071-013SaoPaulo,SPBraziland 3InstituteofMicrobiology,BulgarianAcademyofSciences, Acad.G.BonchevStreet,Building26,1113Sofia,Bulgaria In a new propolis type, red Brazilian propolis, 14 compounds were identified (six of them new for propolis),amongthemsimplephenolics,triterepenoids,isoflavonoids,prenylatedbenzophenonesand a naphthoquinone epoxide (isolated for the first time from a natural source). Three of the major componentsdemonstratedsignificantantimicrobialactivity,andtwo(obtainedasinseparablemixture) possessedradicalscavengingactivityagainst1,1-diphenyl-2-picrylhydrazyl(DPPH). Keywords:antibacterialactivity–chemicalconstituents–propolis–radicalscavengingactivity Introduction red propolis is collected in the North regions. Red colored propolisisreportedtobetypicalforCuba,whereitsplantsource was identified as Clusia nemorosa (Clusiaceae) (13), and for Venezuela,wherebeescollectitfromClusiascrobiculata(14). Inthisstudy,wereportourresultsonantibacterialandantioxid- antactivityofchemicalconstituentsofredBrazilianpropolis. Propolis (bee glue) has a long history of being used as a remedy, dating back to the times of ancient Greece and Rome.Nowadays,itisstillusedforthetreatmentofvarious diseases,and in products like ‘healthfoods’,‘biocosmetics’, etc.,becauseofitsversatilebiologicalactivities(1).Tropical propolis samples, and especially Brazilian ones, have shown significant differences in their chemical composition to propolis from temperate zone (2,3). For this reason, Brazilianbeegl