Biological Effects of Osteoblast-Like Cells on Nanohydroxyapatite Particles at a Low Concentration Range英文文献资料.docVIP

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Biological Effects of Osteoblast-Like Cells on Nanohydroxyapatite Particles at a Low Concentration Range英文文献资料.doc

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Biological Effects of Osteoblast-Like Cells on Nanohydroxyapatite Particles at a Low Concentration Range英文文献资料

HindawiPublishingCorporation JournalofNanomaterials Volume2011,ArticleID104747,6pages doi:10.1155/2011/104747 ResearchArticle BiologicalEffectsofOsteoblast-LikeCellsonNanohydroxyapatite ParticlesataLowConcentrationRange XiaochenLiu, 1JieWei,1,2andShichengWei 1,3 1 2 3 CenterforBiomedicalMaterialsandTissueEngineering,AcademyforAdvancedInterdisciplinaryStudies,PekingUniversity, Beijing100871,China KeyLaboratoryforUltra?neMaterialsofMinistryofEducation,EastChinaUniversityofScienceandTechnology, Shanghai200237,China DepartmentofOralandMaxillofacialSurgery,SchoolofStomatology,PekingUniversity,Beijing100081,China CorrespondenceshouldbeaddressedtoJieWei,nic750@263.netandShichengWei,sc-wei@ Received29April2011;Revised19July2011;Accepted19July2011 AcademicEditor:SomchaiThongtem Copyright?2011XiaochenLiuetal.ThisisanopenaccessarticledistributedundertheCreativeCommonsAttributionLicense, whichpermitsunrestricteduse,distribution,andreproductioninanymedium,providedtheoriginalworkisproperlycited. Thebiologicale?ectsofosteoblast-likeMG-63cellsonnanohydroxyapatite(n-HA)atthelowconcentrationrange(5–25μg/mL) for5dayswasinvestigated.Theresultsshowedtheviabilityandactincytoskeletonofthecellsdescendedwiththeincreaseofthe concentrationofn-HA,andtheactincytoskeletonofcellswasdepolymerisedandbecamemoredisordered.Apoptoticrateofcells (1.85%,1.99%,and2.29%)increasedwiththeincreaseofn-HAconcentration(5,15,and25μg/mL)andbecomesigni?cantly higher than the control. Total intracellular protein content decreased with n-HA concentration increase, showing signi?cant di?erencebetween25μg/mLandthecontrol,andnosigni?cantchangeofALPactivitywasobservedatthe5thday.Theresults revealedthatthecellgrowthwasinhibitedbyn-HAinaconcentration-dependentmanner,andtheobviousbiologicale?ectsof MG-63cellsonn-HAexistedatthelowconcentrationrangefrom5to25μg/mL. 1.Introduction responseston-HAwith theconcentrationof50.8mg/mL) andMotskinetal.(n-HAconcentrationsrangesfrom31to 500mg/mL, and n-HA was toxic at higher concentra