中药活性物质的提纯及抗SARS病毒的研究.pdfVIP

天然产物研究与开发 Nat Prod Res Dev，2007，19：31-33 文章编号：1001-6880(2007)Suppl-0031-03 中药活性物质的提纯及抗SARS病毒的研究 吕海涛 ，潘 娜 ，陈 峰 。山东省青岛农业大学理学院，青岛266109； 莱阳市高级职业学校，莱阳265200； 香港大学植物学系，香港 摘 要：首次利用逆流色谱，对黄芩、甘草和金银花等中药中的生物活性物质进行提取、纯化，黄芩甙、甘草甜素 和绿原酸的纯度分别为96．5％，95．2％和94．8％，通过对10个SAILS冠状病毒临床分离株在胎体恒河猴腰4细 胞和Vero—E6细胞的中和试验和斑块缩小试验，研究三种纯品在培养48和72 h的抗SARS病毒活性。结果表 明黄芩甙抑制SARS病毒活性效果明显，甘草甜素具有一定的效果，而绿原酸的效果较差。 关键词：黄芩甙；甘草甜素；绿原酸；逆流色谱；SARS病毒 中图分类号：R284．1 文献标识码：A Purification of Bioactive Compounds in Traditional Chinese Medicine and Study on Their Effects of Anti-SARS Coronavirus LV Hai—taoh ， PAN Na 。CHEN Feng The College ofScience，Qing~o Agricultural Univemity，Qing~o 266109，China； Laiyang High Vocational School， 265200，China；3Department ofBotany，the University ofHongkong，Hongkong，China Abstract：It WaS the first time to isolate and purify bioactive compounds from Scutellaria baicalensis，Glycyrrhiza uralensis Fisch and Flos lonicerae by high—speed counter—current chromatography(HSCCC)．The purifications of baicalin，glycyr- rhizin and chlorogenic acid were 96．5％ ，95．2％ and 94．8％ ，respectively．The purified compounds were used against 10 isolates of SARS coronavirus seeded with foetal rhesus kidney-4 ceUs and Vero··E6 ceUs at 48 and 72 h by neutraliza·· tion tests with confirmation by plaque reduction assays．It was shown that baicalin has effective activity to inhibit the cy- topathic effect，glycyrrhizin has some activity，and chlorogcnic acid has little activity． Key words：baicalin；glycyrrhizin；chlorogenic acid；high-speed counter-current chromatography；SARS coronavirus 中草药具有明显抑制SARS病毒的作用 工J，金 活性。 银花等六味中草药熬制成的六味合剂，以其对SARS 1 材料与方法 的显著疗