脑慢性低灌注老龄大鼠海马ε型蛋白激酶C及α分泌酶ADAM17表达的变化.pdfVIP

· 330 · Stroke and Nervous Diseases，Dec 2007，Vo114，No．6 ． 脑慢性低灌注老龄大鼠海马￡型蛋白激酶C 及()[分泌酶ADAM 1 7表达的变化 周 燕 闫福岭 【摘要】 目的 观察脑慢性低灌注对老龄大鼠海马组织￡型蛋白激酶C(PKC~)及a分泌酶ADAM17蛋 白表达的影响。方法 将老龄大鼠随机分为假手术组和低灌注组，低灌注组永久性阻断双侧颈总动脉，制作 脑慢性低灌注模型；采用Y型电迷宫分别于术后14和28 d检测大鼠学习记忆能力；采用免疫组化法和免疫 印迹法检测上述时间点海马组织PKC￡和ADAM17的表达。结果 低灌注组术后14和28 d学习记忆能力 较假手术组减退(P0．01)；与假手术组比较，低灌注组术后各时间点大鼠海马PKC￡和ADAM17表达明显 下降(P均0．01)。结论 脑慢性低灌注损害老龄大鼠学习记忆能力，导致痴呆发生，其机制可能与海马 PKCE表达下降有关，而PKC￡参与调节d分泌酶的表达，推测PKC￡表达的下调，导致ADAM17表达减少， 间接促进B淀粉样蛋白(AB)生成，损伤脑组织形成痴呆。 【关键词】 脑慢性低灌注 ￡型蛋白激酶C 分泌酶(ADAM17) 痴呆 【中图分类号1 R741【文献标识码】 A 【文章编号1 1007—0478(2007)06—0330—04 1nhe expression changes of protein kinase C epsilon and tumor necrosis factor-alpha converting enzyme in hip- pocampus of aged rats after chronic cerebral hypopeffusion Zhouyah， Fuling．Department ofNeurology。 Zhongda Hospital A{{tliated to Southeast University，Nan3ing 210009 [Abstractl Objective To explore the effect of chronic cerebral hypoperfusion on the expresstions of pro— tein kinase C epsilon(PKC￡)and tumor necrosis factor-alpha converting enzyme(TACE／ADAM17)in hipp— ocampal of old rats．Methods Wistar rats were divided randomly into two groups：vascular occlusion(2V()) model group and sham-operated group．Permanent bilateral carotid artery occlusion(2V0)models were estab— lished to mimic the chronic cerebral hypoperfusion．Learning and memory abilities in rats were assessed by the Y-maze test at 14 and 28 days after operations．The expression of PKC￡and ADAM17 protein were detected by immuno-histochemistry and western blotting at different times respectively．Results The learning and memory abilities of the