褪黑素对大鼠脑缺血时白细胞浸润和ICAM-1表达的影响.pdfVIP

· 716· J Apoplexy and Nervous Diseases，December 2007，Vol 24，No．6 文章编号：1003—2754(2007)06—071 6-03 褪黑素对大鼠脑缺血时白细胞浸润和 ICAM一1表达的影响 崔利德 ， 韩树斋 ， 李陈莉。 摘 要： 目的 研究褪黑素(melatonin，MT)对大鼠脑缺血损伤缺血区髓过氧化物酶(myeloperoxidase， MPO)的活性及细胞阃黏附分子一1(inte rcellular adhesion molecule一1，ICAM一1)表达的影响。方法 线栓法造成大 鼠大脑中动脉栓塞制成脑缺血模型，手术分为正常对照组、缺血组、MT治疗组，MT治疗组缺血前30min给予MT (20mg／kg)腹腔注射。术后在相应时问点取缺血侧脑组织，用分光光度汁检测MPO的活性，用免疫组化检测I— CAM一1蛋白的表达。结果 与正常对照组比较，缺血组各时问点MPO的活性及ICAM一1蛋白的表达均升高，而与 缺血组比较MT治疗组各时问点MPO的活性及ICAM一1蛋白的表达均降低。结论 MT可能能通过降低MPO的 活性即抑制白细胞的浸润和降低ICAM一1蛋白的表达．对缺血的脑组织产生保护作用。 关键词： 褪黑素； 脑缺血； 髓过氧化物酶； 细胞间黏附分子一l 中图分类号：R965．1 文献标识码：A The effects of melatonin on the activity of myelper0xidase and the expression of ICAM 一1 after focal cerebral is— chemia in rats ( LrI Li—de，HAN Sh zhai，I I(Then—li．(Department f }Anatomy of lining Medical College，。lin— ing 272013，China) Abstract： Objective To study the effects of melatonin(MT)on tbe activity of myeloperoxidase and the ex— pression of interceIlular adhesion molecule一1(ICAM一1)after focal cerebral ischemia in rat’S ischemial area． Methods The cerebral ischemial model was established through a string inserted into the internal carotid artery tO occlude middle cerebral artery．The animals were divided into 3 groups：the normal group，the ischemical group，the melatonin administrated group．Melatonin was administered via peritoneal injection(20mg／kg)30 minute before operation．After operation，the brains were taken at different time points．Spectrophotometry and immunohis— tochemistry chemical method was used for the determination of the activity of MPO and the expression of ICAM一1 in the brain tissue respectively．Results As compared with the control group，1he activity of MPO and the expres— sion of ICAM一1 were increa