苦参碱逆转人肝癌细胞株QGY／CDDP多药耐药性的实验研究.pdfVIP

重庆医科大学学报2008年第33卷第4期 (Journal of Chongqing Medical University 2008．Vo1．33 No．4) 论 薯 文章编号：0253—3626(2008)04-041 1-05 苦参碱逆转人肝癌细胞株QGY／CDDP多药耐药性的实验研究 胥雄阳，何 松 (重庆医科大学附属第二医院消化内科，重庆 400010) 【摘 要】目的：探讨中药苦参碱(Mat)对QGY人肝癌细胞及其耐药细胞株QGY／CDDP的细胞生物学作用和可能的耐药逆转作 用机制。方法：MTY法检测苦参碱对两细胞的半数抑制浓度(Ic 值)并培养Mat作用的QGY／CDDP细胞。比较3组细胞在细胞 生长曲线、倍增时间、细胞周期、化疗药物耐药及逆转和细胞内CDDP含量的差别。免疫细胞化学法测定3组细胞P—gP、MRP、 Bax蛋白的表达。结果：苦参碱可抑制QGY及其耐药细胞株QGY／CDDP的生长，无交叉耐药性。Mat作用的QGY／CDDP细胞与 QGY及其耐药细胞株QGY／CDDP在生物学特性方面表现出一定的差异，细胞的P—gP、MRP、Bax蛋白表达发生变化，降低 QGY／CDDP细胞P—gP、MRP蛋白表达，增加了Bax蛋白的表达，增强CDDP对QGY／CDDP细胞的毒性，其逆转耐药倍数为1．9 倍。结论：低毒剂量的苦参碱可增强CDDP对QGY／CDDP细胞的毒性，具有部分逆转人肝癌耐药细胞QGY／CDDP的耐药作用。 【关键词】肝癌细胞；多药耐药性；苦参碱 【中国图书分类法分类号】R735．7 【文献标识码】A 【收稿日期】2007—10—17 Experimental study of matrine to reverse drug-resistance of human multidrug—resistance hepatOCelIuIar carcinoma celI U Xiong-yang，et al (Department of Gastroenterology，￡胁 Second Affiliated Hospital，Chongqing Medical University) 【Abstract】Objective：To investigate the biologic roles and potential reverse mechanisms of matrine in human hepat~ellular carcinoma cell line QGY and QGY／CDDP．Methods：The MTY assay was used to detect the IC50s of QGY and QGY／CDDP cells，and QGY／CDDP cell were cultivated with matrine．The distinctions of three groups were compared in cell’s appearance， growth curve，doubling time，cell cycle，drug-resistance of chemotherapeutics and CDDP contents of intra—cells． Immun0cytochemical method was applied to detect the expression of P—gp，MRP and Bax．Results： Matrine could inhibit the growth of QGY and QGY／CDDP cells with no cross drug-resistance．There were some biological differences among QGY， QGY／CDDP and Mat—QGY／CDDP cells．The expressions of P—gP and MRP were decreased；Bax expression was increased， CDDP’S toxic effect on QGY／CDDP cells was enhanced，and the drug sensitivity