中药提取物对油菜菌核病菌抑制活性及丁香活性成分的研究.pdfVIP

安徽农业科学，Journal ofAnhui agn．Sci．2008，36(4)：1484—1485，1510 责任编辑 庆珞 责任校对 马君叶 中药提取物对油菜菌核病菌抑制活性及丁香活性成分的研究 陈浩 ，宁睿 ，苏兴海2，李优琴 ，王春梅 ，石志琦 (1．江苏省农业科学院食品安检所，江苏南京210014；2．江苏省海安县胡集镇农业技术推广站，江苏南通226671) 摘要 [目的]对中药提取物进行抑菌成分分析，为新型植物源农药的研制提供基础。[方法]以丁香等14种中药乙醇提取物为材料，测 定各提取物对油菜菌核病菌的抑制活性，采用气相色谱一质谱联用法分析丁香提取物的化学成分，并测定其对油菜菌核病菌抑菌活性和 油菜菌核病菌菌丝干重的影响。[结果]结果表明：丁香提取物对油菜菌核病菌菌丝生长抑制效果最好，抑制率为100％。[结论]初步证 实丁香酚为丁香提取物的主要抑茵成分。 关键词 抑真菌活性；中药提取物；油菜菌核病菌；丁香酚 中图分类号 $481 ．9 文献标识码 A 文章编号 0517—6611(2∞8)04—01484—02 Studies 011 the Inhibitory Activity of Extracts of Oainese Herbs to Sc／erot／n／a sclerotiorum inIpe and Active Components in Sy~／u,n aro- mal／cum L． a I-lao et al (Institute of Food Security Inspection，Jiallgsu Academy of Agrieuhural Sciences，Nanjing，Jiallgsu 21OO14) Allstrad [Objective]1he aim of the research WaS to analyze the antifungal components of Chinese herb extracts and provide basis f0r t}le development of new types of plant pestieides．[Methodj With the ethanol extracts from 14 kinds of Chinese herbs including ∽ aromat／cum L．a．s materials，the inhibitory activities ofall kinds of exffaets to Sclerotinia sclerotiorum in rape were determined 1he chemical eomt~nents in the extracts from Js．n，D— mat／cure were analyzed bv GC—MS method andits effects on the antifungai activity to S．sclerotiorl1171 in rape and the mycelium drv weight of Js．sclerotio— M were detennined．1 Resultl The results showed that the inhibitory effects ofthe ex~acts from S．aromat／cum on the mycelium growth of Js．selerotio— M in rapewere better andtheinhibitory ratewas100％~．[Conclusion]Eugenolwas preliminarily verifiedtobemain antifungal eortrt~onentinthe extracts from S．aromat／cum． Key words Antifungal activi~；Chinese herb extracts；Sclerotinia sclerotiorum in rape；Eugenol 油菜菌核病菌(Sderotinia sclerotiorum)引起的油菜菌核病 设备的样品杯中，加95％乙醇溶液浸泡过夜后进行连续循环 在我国主要发生于长江流域和东南沿海各省，高发年份一些 提取，15 rnjr 循环，共20个循环，提取温度11℃，提取完成后 田块的油菜产量损失达8