丰富与贫瘠环境对新生鼠脑损伤后脑功能的影响.docVIP

编号： 刊用形式： 文章编号： 丰富与贫瘠环境对新生鼠脑损伤后脑功能的影响 鲁利群 江俊 杨欣 刘松梅 （成都医学院第一附属医院儿科，四川成都 610500） 提 要： 目的 研究丰富与贫瘠环境对缺氧缺血性脑损伤（hypoxic- ischemic brain damage, HIBD）后脑功能的影响。方法 制备3日龄SD大鼠HIBD模型，随机分为丰富环境组（enriched environment, EE）impoverished environment, IE）standard environment, SE)。另设假手术组(Sham)对照。各组大鼠于术后第2d开始分别给予不同环境刺激。术后第32d，应用悬吊和斜坡实验测试感觉运动功能，Morris水迷宫实验了解学习记忆能力，免疫组织化学染色检测皮层和海马胶质纤维酸性蛋白(Glial fibrillary acidic protein, GFAP）的表达。结果 感觉运动和学习记忆能力测试发现，IE组明显差于SE组（P0.01），SE组差于Sham组和EE组（P0.01），Sham组和EE组无显著差异（P0.05）。皮层和海马GFAP免疫组织化学染色结果显示，Sham组GFAP阳性细胞数分别低于其它三组（P0.01），而SE组低于EE组，高于IE组（P0.01）。结论 丰富环境增加新生鼠脑损伤后GFAP的表达，改善脑功能；贫瘠环境加重脑功能的损害。 关键词：缺氧缺血；环境；胶质纤维酸性蛋白；脑功能；早产 Effects of enriched environment and impoverished environment on brain function of premature rats after hypoxic- ischemic brain damage LU Li-qun, JIANG Jun, YANG Xin, LIU Song-mei （Department of Pediatrics, the First Affiliated Hospital of Chengdu Medical College, Chengdu, 610500, China） Abstract：Objective To the effects of enriched environment and impoverished environment on brain function after hypoxic-ischemic brain damage (HIBD). Methods SD rats of HIBD models at postnatal days 3 were established at first, then they were divided randomly into enriched environment stimulation group(EE), standard environment stimulation group (SE), and impoverished environment group (IE). The sham-operation rats served as control group. Different environment stimulation was administrated to the rats respectively since the 2nd day after HIBD.On the 32nd day, movement (hanging test and incline plane test) was used to evaluate the sensorimotor function, Morris water maze was used to evaluate the learning and memory ability. The expression of GFAP in cortex and hippocampus was measured by the method of immunohistochemistry. Results The brain function of IE group was much lower than that of SE group（P0.01）, and the function of SE group was lower than that of sham group and EE group（P0.01）. There was no signi