骨髓间充质干细胞对大鼠皮层神经元缺氧缺糖损伤后轴突生长和AKTGSK-3βCRMP-2表达的影响.pdfVIP

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骨髓间充质干细胞对大鼠皮层神经元缺氧缺糖损伤后轴突生长和AKTGSK-3βCRMP-2表达的影响.pdf

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骨髓间充质干细胞对大鼠皮层神经元缺氧缺糖损伤后轴突生长和AKTGSK-3βCRMP-2表达的影响

1 目录英文缩略词表 ·····················································3 中文摘要·························································4 英文摘要·························································6 前言···························································8 第一部分骨髓间充质干细胞和大鼠皮层神经元的分离培养及鉴定引言·························································10 材料与方法···················································10 结果·························································15 讨论·························································17 第二部分骨髓间充质干细胞与缺氧缺糖损伤后的神经元共培养模型的建立引言·························································19 材料与方法···················································19 结果·························································21 讨论·························································22 第三部分骨髓间充质干细胞对神经元缺氧缺糖损伤后轴突的生长及蛋白 pAKT 、pGSK-3β、pCRMP-2 、GAP-43 表达的影响引言·························································24 材料与方法···················································24 结果·························································29 讨论·························································32 结论·····························································34 论文参考文献·····················································35 致谢···························································40 综述···························································41 综述参考文献·····················································46 2 英文缩略词表(Abbreviations and acronyms) 英文缩写英文全称中文全称 BMSCs Bone marrow-derived mesenchymal stem 骨髓间充质干细胞 cells B27 B27 serum-free supplemen