genistein on human pituitary adenoma cell proliferation（染料木黄酮在人垂体腺瘤细胞增殖）.docVIP

下载本文档

0
0
约1.66万字
约 16页
2017-09-06 发布于浙江
举报
版权申诉

genistein on human pituitary adenoma cell proliferation（染料木黄酮在人垂体腺瘤细胞增殖）.doc

1、原创力文档（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。。
2、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击这里二次下载。
3、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
4、该文档为VIP文档，如果想要下载，成为VIP会员后，下载免费。
5、成为VIP后，下载本文档将扣除1次下载权益。下载后，不支持退款、换文档。如有疑问请联系我们。
6、成为VIP后，您将拥有八大权益，权益包括：VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
7、VIP文档为合作方或网友上传，每下载1次，网站将根据用户上传文档的质量评分、类型等，对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档

genistein on human pituitary adenoma cell proliferation（染料木黄酮在人垂体腺瘤细胞增殖）

Genistein on human pituitary adenoma cell proliferation Author: Chi Sumin Zhoushi Sheng Liu Yali Jian-Wen Gu Wang Fuzhou [Keywords] genistein Keywords: genistein; pituitary adenoma cells; cultured in vitro Abstract: Objective To observe genistein (genistein, the impact of the GST on the proliferation of cultured human pituitary adenoma cells. Methods GST role in cultured human pituitary adenoma cells measured MTT value and 3 H-TdR intake and streaming cytometry to determine the cell cycle. Results of different concentrations of GST (10-7 ,10-6 and 10-5 mol ?L-1 significantly inhibited the proliferation of pituitary adenoma cells A490nm value of the rate of change of the MTT, respectively (91.2 + -4.9 %), (78.8 + -5.3% (65.8 + -5.6% (P lt;0.01, the rate of change of the CPM value was (88.6 + -3.6% (75.5 + -5.7%) and (58.9 + -4.9% (P lt;0.01, and there are dose-response .10-5 mol ?L-1, GST will enable the proportion of cells in G1 phase increased to 91.0% from 68.2% in the control group (P lt;0.01. genistein on pituitary adenoma cells. the role of estrogen receptor. conclusion GST could significantly inhibit the in vitro proliferation of cultured human pituitary adenoma cells. Keywords: genistein; pituitary adenoma cells; in vitro Abstract: AIM To study the influence of genistein on the proliferation, DNA synthesis and cell cycle of cultured human pituitary tumors cells.METHODS MTT method and3 H-TdR incorporation cell cycle analysis were used to examine the changes of proliferation and DNA synthesis of human pituitary tumors cells.RESULTS In a dose dependent man-ner, genistein at different concentration (10-7 mol ?L-1 ,10-6 mol ?L-1 ,10-5 mol ?L-1 could significantly inhibit the proliferation of human pituitary adenoma cells cultured in vitro.The change rate of A490nm value were (91.2 + -4.9)%, (78.8 + -5.3% and (65.8 + -5.6% respectively (P lt;0.01, and the change rates of cpm value were (88.6 + -3.6%, (75.5 + -5.7% and (58.9 + -4.9)% respecti