产β-环糊精葡萄糖基转移酶高温菌株hy15发酵条件优化 optimization on fermentation conditions of β-cyclodextri glucanotransferase producing thermophiles hy15.pdfVIP

V01．32NO．10 2013 China ·40· SerialNo．259 Brewing Research Report 产卢一环糊精葡萄糖基转移酶高温菌株HYl5发酵条件优化 曹冬梅，，．，胡耀辉- 培养温度、MgS04·7H20等对酶活力影响。并设计正交试验优化其发酵条件。结果表明最优的发酵条件为：碳源为玉米淀粉+糖蜜，氮 7／mI，。 关键词：_B一环糊精葡萄糖基转移酶；发酵条件；优化 中图分类号：Q93—335 文献标识码：A 文章编号：0254—5071(2013)10—0040—04 onfermentationconditions Optimization of／3-cyclodextriglucanotransferase HYl5 producingthermophiles CAO Yaohuil Dongmeiu，HU ofFoodScience 130118,Ctdna； (1．College andEngineering,JilinAgffculmralUniversity,Changchun 2．CollegeofFood,HeilongfiangBayiAgriculturalUniversity,Daqg163319,China) HYl5wasstudiedonitsfermentationconditionsinthis Abstract：A卢一cyclodextriglucanotransferase(B-CGTase)producingthermophiles paper．Or- basedon factortestswas to theeffectsofcarbon thogonaldesign single appliedstudy source，nitrogensource，initialpHvalue，culturetemperature and on resultsshowedthatthe fermentionconditionswere molassesandcornstarchascarbon MgS04·7H20enzymeactivity．The optimum using as value ofshaker source，cornsteepliquornitrogensource，initialpH 7．0，fermentationtemperature60℃，rotationspeed undertheseconditions． couldbe t01794．3U／ml activity up words：lIB—cyclodextringlucanotransfcmse；fermentationconditions；optimization Key