超声波预处理醇碱体系进行甲壳素脱乙酰工艺的研究 study on the deacetylation technology of chitin in ethanol-sodium hydroxide system under ultrasonic pretreatment.pdfVIP

现代食品科技 Modern Food Science and Technology 2011, Vol.27, No.4 超声波预处理醇碱体系进行甲壳素脱乙酰工艺的研究 侯佰立，吉宏武 （1.水产品深加工广东普通高校重点实验室，广东湛江 524088） （2.广东海洋大学食品科技学院，广东湛江 524025） 摘要：以自溶法脱蛋白、EDTA⋅Na 螯合脱钙制备的甲壳素为原料，采用超声波预处理醇碱体系进行甲壳素脱乙酰工艺的研究。 2 以脱乙酰度（D.D. ）为考察指标，依据单因素实验结果，采用L 4 9(3 )正交试验考察乙醇浓度（A ）、氢氧化钠质量浓度（B ）、反应时间 （C ）及超声波处理时间（D ）对脱乙酰度（D.D. ）的影响。实验结果表明脱乙酰工艺的最佳参数为：超声波预处理 3 h；乙醇浓度 55%；NaOH 质量浓度 28%；反应时间 8 h。在此条件下，甲壳素溶解率 1%，壳聚糖脱乙酰度87.62%。 关键词：甲壳素；壳聚糖；超声波；醇碱体系；参数优化 文章篇号：1673-9078(2011)4-418-422 Study on the Deacetylation Technology of Chitin in Ethanol-sodium Hydroxide System under Ultrasonic Pretreatment HOU Bai-li, JI Hong-wu (1.Key Laboratory of Aquatic Product Advanced Processing of Guangdong Higher Education Institutes, Zhanjiang 524088, China) (2.College of Food Science Technology, Guangdong Ocean University, Zhanjiang 524025, China) Abstract: Deacetylation technology of chitin in ethanol-sodium hydroxide system under ultrasonic pretreatment with chitin as raw material by autolysis deproteinization and EDTA· Na2 chelating decalcification methods was studied in the issue. On the basis of single-factor tests, deacetylation degree (D.D.) was used to evaluate the performance of ethanol concentration(A), sodium hydroxide concentration (B), reaction time (C) and time of ultrasonic pretreatment (D) on deacetylation degree (D.D.) by using L 4 9(3 ) orthogonal experiment design . The optimum deacetylation conditions were as follows: time of ultrasonic pretreatment 3 h, ethanol concentration 55 %,