钙离子诱导大鼠肾小管上皮细胞氧化应激损伤的研究.docVIP

精品论文 参考文献 钙离子诱导大鼠肾小管上皮细胞氧化应激损伤的研究 王扬 黎承杨（通讯作者） 　　（广西医科大学研究生学院 广西 南宁 530021） 　　【摘要】 目的：观察体外培养条件下大鼠肾小管上皮细胞在钙离子诱导下的氧化应激损伤的情况。方法：体外培养大鼠肾小管上皮细胞，使用含钙钙离子的培养液作为诱导剂，实验共分为三组：(1)正常对照组（+正常培养液）；（2）Ca I组（+10mmol/L Ca2+液）；（3）Ca II组（+20mmol/L Ca2+液）。各组细胞分别培养至6h、12h、24h时，采用MTT法检测细胞增殖活性。培养12h时收集细胞培养上清液，采用化学比色法分别检测各组细胞上清液过氧化氢（H2O2）和丙二醛（MDA）的浓度。结果：结果显示细胞的抑制率随着钙离子浓度的增加而增加。对照组、Ca I组、Ca II组的细胞上清液H2O2浓度分别是7.40plusmn;1.24mmol/L, 18.20plusmn;0.96mmol/L, 21.34plusmn;1.23mmol/L。经统计学分析，各组差异有统计学意义（P＜0.05)。对照组、Ca I组、Ca II组的细胞上清液丙二醛（MDA）浓度分别是30.41plusmn;2.33nmol/L, 56.45plusmn;4.38nmol/L, 67.57plusmn;3.89nmol/L。经统计学分析，各组差异有统计学意义（P＜0.05)。结论：本研究的结果提示：钙离子能诱导大鼠肾小管上皮细胞出现氧化应激损伤。 　　【关键词】 大鼠肾小管上皮；钙离子；H2O2；MDA；氧化应激 　　【中图分类号】R965 【文献标识码】A 【文章编号】2095-1752（2016）09-0026-02 　　Study of oxidative stress injury of rat renal tubular epithelial cell induced by calcium Wang Yang 　　Guangxi Medical University, Guangxi, Nanning 530021, China 　　【Abstract】Objective To investigate the oxidative stress injury of calcium on the rat renal epithelial cells (NRK52E) in vitro. Methods Cultured rat renal epithelial cells (NRK52E) were divided into 3 groups in this study:(1) the control group (+normal media); (2) CaⅠgroup (+10mmol/L Ca2+ media); (3) CaⅡgroup (+20 mmol/L Ca2+ media) . The cells were incubated with or without additives for 6 h、12 h and 24 h . The cell activity of each groups were measured by the MTT at 6 h、12 h、24 h, respectively. At 12 h, the production of H2O2 and MDA in the media were measured by chemical colorimetry. Results MTT test showed that the cell proliferation inhibition rate increased significantly with the increase of calcium concentration in media. The concentrations of H2O2 in the media of the control, Ca I, Ca II are 7.40plusmn;1.24mmol/L, 18.20plusmn;0.96mmol/L, 21.34plusmn;1.23mmol/L. The concentrations of H2O2 in the media of the control, Ca I, Ca II are 30.41plusmn;2.33nmol/ml, 56.45plusmn;4.38nmol/ml, 67.57plusmn;3.89nmol/ml. Conclutions our stud