肥胖zucker大鼠肠系膜动脉多巴胺d1受体介导血管舒张功能受损及其机制-impaired vasodilation function mediated by dopamine d1 receptor in mesenteric artery of obese zucker rats and its mechanism.docxVIP

缩略语表缩写 英文全称 中文全称MS Metabolic Syndrome 代谢综合症Fen Fenoldopam 非诺多泮ROG Rosiglitazone 罗格列酮STZ Streptomycin 链霉素HFD High-fat diet 高脂饮食PSS Physiological salt solution 生理盐溶液KPSS High-potassium PSS 高钾盐溶液Ach Acetylcholine 乙酰胆碱PHE Phenylephrine HCl 盐酸苯肾上腺素SNP Sodium nitroprusside 硝普钠PBS Phosphate buffered saline 磷酸盐缓冲液FBS Fetal bovine serum 胎牛血清SDS Sodium dodecyl sulfate 十二烷基硫酸钠PAGE Polyacrylamide gelelectrophoresis 聚丙烯酰胺凝胶电泳PBST Phosphate buffered saline with Tween 20 含有吐温20的磷酸盐缓冲液 A10 Embryonic thoracic aortic smooth muscle cells from rats 大鼠胚胎胸主动脉平滑肌细胞 MAPK Mitogen-activated protein kinase 丝裂原活化蛋白激酶IRS Insulin Receptor Substrate 胰岛素受体底物PI3K Phosphatidyl Inositol 3-Kinase 磷脂酰肌醇3-激酶PKB protein kinase B 蛋白激酶 BELISA Enzyme-linked immuno sorbent assay 酶联免疫吸附实验WB Westen Blot 免疫印记Co-IP Co-immunoprecipitation 免疫共沉淀PMSF Phenylmethanesulfonylfluoride 苯甲基磺酰氟Tris-HCl Trishydrochloric acid buffer solution Tris-盐酸缓冲液RAAS Rennin-angiotensin-aldosterone system 肾素-血管紧张素-醛固酮系统NC Nitrocellulose 硝酸纤维素1PD98059 Inhibitor of MAPKsMAPK/ERK 抑制剂SCH23390 Inhibitor of D1 receptorD1 受体拮抗剂EEndothelium内皮细胞rpmrevolutions per minute转/分hrhour小时minminute分钟Mmol/L摩尔/升℃Centigrade摄氏度KDKilo-doltons千道尔顿mmHgMillimeters of mercury毫米汞柱PP-ValueP 值2Impaired dopamine D1 receptor-mediated vasorelaxation of mesenteric arteries in obese Zucker ratsAbstractObjective：Obesity and hypertension are two important components of the metabolic syndrome, often coexist in the same individual, and promote the occurrence and development of cardiovascular disease [ 1][2]. The reason about obesity-related hypertention is not fully understood. One of the possible etiologies of the metabolic syndrome is insulin resistance [1][2][3][4]. Insulin stimulated sympathetic nerve system activity[5], vascular smooth muscleproliferation[6]and sodium and water retention[7]and the inhibitory effect on prostacyclinsynthesis[ 8]have been suggested to be involved in the pathogenesis of obesity-