几丁糖对扩张后皮瓣纤维包膜影响临床研究.docVIP

几丁糖对扩张后皮瓣纤维包膜影响临床研究 　　[摘要]目的：探讨几丁糖干预纤维包膜形成的作用机理。方法：共对24例患者行皮肤软组织扩张术，每个患者均在病变周围两侧各埋植100ml皮肤软组织扩张器一枚；将其中一侧设为治疗组，将2%医用几丁糖2ml注入腔隙内，然后植入100ml皮肤软组织扩张器；另一侧设为对照组腔隙剥离完毕后观察无活动性出血，直接植入100ml皮肤软组织扩张器。分别制作HE染色、Masson 染色、CD34免疫组化染色切片进行相关观察；并随机选取每组各15个视野应用显微标尺测量纤维包膜厚度，同时标本做电镜观察。结果：治疗组纤维包膜厚度532.500±112.033μm，对照组897.000±206.023μm两组比较有统计学差异（P 　　[关键词]几丁糖；纤维包膜；皮肤软组织扩张术；组织学 　　[中图分类号]R622[文献标识码]A[文章编号]1008-6455（2011）07-1090-04 　　 　　Clinical Application and Research of Chitosans effect on the flap of fibrous capsule 　　GUO Wen Zhe1,QIU Shu-lin2,YAN Yan3,CHU Guo Hua4,CUI Guo Sheng5,ZHANG Zhi-xin1,ZHANG Shu-xiang1 　　(1.Department of Plastic Surgery, AffiliatedHospital to Hebei University,Baoding 071000,Hebei,China；2. Department of Plastic Surgery,Hebei Peoples Hospital,Shijiazhuang 050051,Hebei,China；3. DepartmentofENT Electric Power Hospital of Beijing；4. DepartmentofPlastic Surgery,The Second Central Hospital of Cangzhou) 　　 　　Abstact:ObjectiveTo probe into the mechanism that how chitosan can intervene the formation of fibrous capsule.MethodsA total of 24 cases of patientshave had skin and soft tissue expansion,the 100ml one skin and soft tissue expander were implanted around both sides of the lesions of each patient,set the treatment group on one side,inject2ml 2% Chitosaninto the inner compartment,and then implanted 100ml skin and soft tissue expander; the other side is seted as control group, after stripping the compartment no active bleeding observed, then implant 100ml skin and soft tissue expander directly. Produced a HE staining,Masson staining, CD34 immunohistochemical staining for the relevant observation; and selected 15 visions in each group randomly with theapplication ofmicro-scale to measure the thickness of the fibrous capsule, and use electron microscopy to observe the specimens. ResultsThickness of the fibrous capsule of the treatment group was 532.500 ± 112.033μm,the control group was 897.000 ± 206.023μm, there were significant differences between the two groups(P