运动性疲劳影响大鼠学习记忆功能和脑组织内Tau表达.docVIP

运动性疲劳影响大鼠学习记忆功能和脑组织内Tau表达 　　摘 要：目的：建立大鼠运动性疲劳模型，通过免疫组织化学法检测大鼠大脑皮层和海马CA1区内Tau蛋白的分布与表达，探讨其在运动性疲劳致大鼠学习记忆障碍发病机理中的作用。方法：成年雄性Wistar大鼠随机平均分为对照组、疲劳即刻组和疲劳恢复90 min组。疲劳即刻组和疲劳恢复90 min组大鼠进行十天游泳运动，每天运动至力竭，对照组大鼠不加干预。运用避暗穿梭实验检测大鼠学习记忆功能损伤情况，免疫组织化学法检测大脑皮层和海马CA1区Tau蛋白分布及表达，并测定Tau蛋白免疫阳性细胞的积分光密度值（IOD）。结果：疲劳即刻组和疲劳恢复90 min组大鼠学习记忆功能有不同程度损伤，其大脑皮层和海马CA1区Tau蛋白表达较对照组上调(P 　　关键词：运动性疲劳；学习；记忆；Tau 　　中图分类号：G804.7文献标识码：A文章编号：1007-3612(2011)06-0054-03 　　 　　Effects of Exercise-induced Fatigue on Rat Learning and Memory Ability and Expressions of Tau in the Brain 　　 　　WANG Tai-lin 　　(Sports and Art Institute, North University of China, Taiyuan 030051, Shanxi China) 　　 　　Abstract:Objective: Through establishing the rat model of exercise-induced fatigue，the paper detected the distribution and expression of Tau in the cerebral cortex and hippocampus CA1, and explored the role of Tau in fatigue-induced learning and memory disorder. Methods: Adult male SD rats were randomly divided into control group, 10-day fatigue group and recovery 90min group．Rats in 10-day fatigue group and recovery 90min group exercised to exhausted by swimming for 10 days，and those in control group didn’t exercise．Dark avoidance test was used to detect the changes of learning and memory ability．Immunohistochemistry was used to observe the distribution and expressions of Tau in the cerebral cortex and hippocampus．IOD of Tau- immunoreactive cells were also countered．Results: Rats with exercise-induced fatigue showed learning and memory disorder，and in the cerebral cortex and hippocampus CA1 of rats in the fatigue group，the expressions of Tau increased obviously compared with that of the control group(P 　　1.3 避暗穿梭实验 采用上海移数信息科技有限公司生产的避暗穿梭测试仪，该测试仪有明、暗两个箱子，箱与箱之间有隔板分??，在底部有一个孔洞可以使明暗箱互通。把大鼠放入明箱适应30 s后，打开隔板，大鼠会通过孔洞钻入暗箱。暗箱底部有带电钢棍，等大鼠身体全部钻入暗箱时立即施加电击，从而使其获得记忆。24 h后再将大鼠放入明箱，记录大鼠自放入明箱到第一次钻洞进入暗箱的时间，该时间被称为潜伏期，及进入暗箱的错误次数。检测时间以300 s为限，如果300 s内仍不进入暗箱者，视为该大鼠的记忆保持良好。 　　1.4 Tau蛋白的免疫组织化学实验 运动性疲劳后大鼠用戊巴比妥钠腹腔麻醉，经心脏快速灌注冲洗生理盐水约250 mL，再用4％多聚甲醛