神经鞘磷脂合成酶2基因缺失可以减慢糖尿病进程-deletion of sphingomyelin synthase 2 gene can slow down the progression of diabetes.docxVIP

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神经鞘磷脂合成酶2基因缺失可以减慢糖尿病进程-deletion of sphingomyelin synthase 2 gene can slow down the progression of diabetes.docx

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神经鞘磷脂合成酶2基因缺失可以减慢糖尿病进程-deletion of sphingomyelin synthase 2 gene can slow down the progression of diabetes

HOMA-IR: The HOMA-IR was significantly higher in WT mouse fed with high-calorie diet for 2 months than before fed[(4.11±0.50)vs(3.32±0.48)，p0.05 ], in SMS2-/- mouse fed high-calorie diet for 3 months than before fed[(4.26±0.72)vs(2.95±0.74)，p0.05 ]. Plasma SM: The plasma SM in SMS2-/- which fed with high-calorie diet was significantly lower than that in WT group[(3.87 ± 0.45) mg · dl-1vs (4.26 ± 0.44) mg · dl-1, p 0.05]. ITT: The glucose metabolic clearance rate of SMS2-/- group was 1.31 times as high as that of WT. IPGTT: Before the feeds, two groups of mice were normal in glucose tolerance. After 3 months, 2hPG glucose load were significantly higher than fasting blood glucose in WT group [(2.22±0.17) mmol·l-1 vs (1.86±0.22) mmol·l-1, p 0.05], impaired glucose tolerance damage, however SMS2-/- group with normal glucose tolerance. IRT: The insulin level didn’t appear significant peak time in 1 hour in WT group after glucose were loaded and this showed dysinsulinism. The insulin level decreased in 15 min and rose by an average of 3.4 in 30-60min in SMS2-/- group after glucose were loaded which showed insulin secretion is normal. Linear regression analysis: The plasma SM densities in WT group and SMS2-/- group both influenced the prophase blood glucose levels after glucose load from regression equation. Adipocyte: After stained by hematoxylin-eosin, microscopic imaging calculated surface area of epididymal adipocyte. Compared with WT group, the SMS2-/- group has prominently less area [ (343.74±50.44)μm2 vs (670.88±54.65) μm2 ，p0.01]. Conclusions: WT group: With high-calorie diet feedings for 3 months, mice became fat together with glucose tolerance damaged. And peripheral insulin sensitivity decreased.. SMS2-/- group: With high-calorie diet feedings for 3 months, mice lost weight, the glucose tolerance was normal, insulin secretion was normal and insulin sensitivity increased. To sum up the above arguments, WT mice would have severe T2DM with further d