MTH1与乳腺肿瘤相关性研究.docVIP

MTH1与乳腺肿瘤相关性研究 　　[摘要] 目的 研究MTH1?c乳腺肿瘤的相关性。方法 选取2014年5月～2015年7月新乡市中心医院57例乳腺蜡块及部分组织。按乳腺良性肿瘤和乳腺恶性肿瘤分成两组，分别为20例和37例。通过免疫组化的方法检测两组组织实质、间质中MTH1的含量。将恶性肿瘤组织按激素受体（HR）（HR包括雌激素和孕激素受体）阴性、阳性；淋巴结有无转移；Ki-6720%、≤20%；人表皮生长因子受体2（HER-2）（-）、HER-2（+～+++）分别分两组，通过免疫组化方法检测各组实质、间质中MTH1的含量。进一步应用Western blot方法检测乳腺肿瘤组织中MTH1的表达差异。结果 MTH1在乳腺肿瘤实质及间质中的含量，恶性肿瘤明显高于良性肿瘤，差异有统计学意义（P 0.05）；MTH1在有淋巴结转移和无淋巴结转移的乳腺恶性肿瘤组织中含量差异无统计学意义（P 0.05）；MTH1在乳腺恶性肿瘤实质及间质中的含量，Ki-6720%组中明显高于Ki-67≤20%组，HER-2（+～+++）组中明显高于HER-2（-）组，差异有统计学意义（P 0.05）。结论 乳腺恶性肿瘤组织中MTH1明显高于乳腺良性组织，并且恶性程度与MTH1含量呈正相关。 　　[关键词] 乳腺恶性肿瘤细胞；乳腺良性肿瘤细胞；MTH1；免疫组化 　　[中图分类号] R737.9 [文献标识码] A [文章编号] 1673-7210（2017）01（b）-0102-04 　　Study on the correlation between MTH1 and mammary tumor 　　DU Xueqian1 ZHOU Yong2 QI Pan2 YUAN Longjian1 HENG Ruijuan1 YANG Liuqin2 　　1.Xinxiang Medical University， Henan Province， Xinxiang 453000， China； 2.Department of Oncological Surgery， Xinxiang Central Hospital， Henan Province， Xinxiang 453000， China 　　[Abstract] Objective To study the correlation between MTH1 and mammary tumor. Methods Fifty-seven paraffin-embedded samples of breast and some breast tissue obtained from Xinxiang Central Hospital from May 2014 to July 2015 were selected. They were divided into two groups， including mammary benign tumor and mammary malignant tumor， which contained 20 cases and 37 cases respectively. The content of MTH1 in breast parenchyma and mesenchyme was assayed by immunohistochemical staining. The malignant tumor group was divided into two groups respectively based on positive or negative hormone receptor （HR） （HR included estrogen receptor and progestrone receptor）， lymphatic metastasis or not， Ki-6720% or ≤20%， human epidermal growth factor recptor 2 （HER-2） （-） or HER-2 （+-+++）， the content of MTH1 in breast parenchyma and mesenchyme was assayed by immunohistochemical staining. Western blot was used to detect the expression of MTH1 in breast tumor tissues. Results For the content of MTH1 breast parenchyma and mesenchyme， the mammary