p73基因在急性白血病中表达及临床意义.docVIP

p73基因在急性白血病中表达及临床意义 　　[摘要] 目的 探讨p73基因在急性白血病(AL)中的表达及其临床意义。方法 采用RT-PCR方法检测100例初诊及复发或难治AL患者和20例正常对照者p73基因的表达,并采用χ2检验、t检验及单因素方差分析对结果进行统计学分析,以比较各组p73基因表达阳性率及表达强度差异。结果 58%的AL患者有较高水平的p73的表达,初诊及复发或难治组明显高于经过治疗缓解组及正常对照组(P0.05)。结论 p73基因表达在AL的发病机制及不良预后中起了重要的作用。 　　[关键词] 急性白血病; p73; 发病机制; 预后 　　[中图分类号] R733.71 [文献标识码] A [文章编号] 1673-9701(2010)09-01-03 　　 　　Expression of p73 Gene in Acute Leukemia and Its Clinical Significance 　　LI Qianru1# SU Liping2 WU Jianrui3 ZHAO Jin2 SHI Lei4 LI Mingli1 ZHENG Meijing1 DUAN Jingjing1 ZHOU Yongan5* 　　1.Shanxi Medical University,Taiyuan 030000,China;2.Shanxi Province Tumor Hospital,Taiyuan 030000,China;3.Shanxi Province Childrens Hospital,Taiyuan 030000,China;4.The Second Hospital of Shanxi Medcical University,Taiyuan 030000,China;5.Taiyuan Central Hospital,Taiyuan 030000,China 　　 　　[Abstract] ObjectiveTo study the p73 gene expression in acute leukemia(AL) and its clinical significance. MethodsThe p73 gene ex- pression in 100 cases of AL including newly diagnosed and recrudescent or refractory ones and 20 normal controls was detected by relative quantitative reverse transcriptase polymerase chain reaction(RT-PCR),and then the results were analyzed and compared by χ2-test,t-test and single agent variance. ResultsThe higher expression of p73 gene was found in 58% of AL cases. The expression level of p73 gene in newly diagnosed and recrudescent or refractory cases was significantly higher than that of the relief cases after therapy and than that of the controls(P0.05). ConclusionThe expression of p73 gene plays an important role in the pathogenesis and poor prognosis of AL. 　　[Key words]Acute leukemia; P73; Pathogenesis; Prognosis 　　 　　p73基因是1997年由法国的Kaghad等[1]从神经母细胞瘤细胞系中克隆出来的具有抑癌作用的基因。p73基因是p53基因家族的新成员,具有细胞周期调节及诱导细胞凋亡的作用,但是与p53基因在人类肿瘤发生中经常发生突变不同,至今极少发现p73有突变,而且p73基因在不同肿瘤中表达水平差异很大,显示了抑癌基因和癌基因的双重特性[2]。我们采用RT-PCR方法检测了AL患者骨髓单个核细胞p73 mRNA的表达,并分析了p73 mRNA表达与诱导缓解化疗的疗效及复发的关系,探讨p73基因与AL的发生、发展及预后的关系。 　　1 资料与方法 　　1.1 研究对象 　　2008年6月～2009年3月期间在