TP在原发性肝细胞癌中表达的研究及临床意义-外科学(普外)专业论文.docxVIP

摘要 摘要 万方数据 万方数据 摘 要 目的： 研究 TP（腺嘧啶脱氧核苷磷酸化酶）蛋白在原发性肝细胞癌(hepatocellular carcinoma, HCC)中癌组织、癌旁组织、门静脉癌栓的表达，进一步研究分析微 血管密度(microvessal density，MVD)、癌旁组织 TP 与临床病理特征之间的关系， 分析癌旁组织 TP 蛋白与 MVD 的相关性，探讨 TP 在原发肝细胞癌转移治疗中 的潜在价值。 方法： 标本取自海南医学院附属医院确诊原发肝细胞癌患者80例，年龄30-76岁， 平均52岁。应用免疫组织化学法检测肝癌组织、癌旁组织、门静脉癌栓的 TP 蛋白的表达，对 TP 表达的强弱进行测定，以抗 CD34标记分析癌旁组织 MVD， 并结合临床资料进行统计学分析。 结果： 1、TP 蛋白在肝癌旁组织和门静脉癌栓中表达明显高于肝癌组织(P0.05)。 2、肝癌旁组织 TP 的表达与 Child-Pugh 分级、肿瘤包膜是否侵犯、肿瘤大 小、有无门静脉癌栓、是否肝硬化明显相关(P0.05)；而与性别、年龄、甲胎蛋 白无关(P﹥0.05)。 3、肝癌旁组织 MVD 与肝癌分化程度、Child-Pugh 分级、是否肝硬化相关 (P0.05)；而与年龄、性别、甲胎蛋白及肿瘤的大小无关(P﹥0.05)。 4、肝癌旁组织 TP 蛋白表达与 MVD 正相关。 结论： 1、TP 蛋白在肝癌旁组织中表达明显增加，在门静脉癌栓中高表达，为靶 向药物卡培他滨参与治疗肝癌的术后转移提供一定的实验依据。 2、肝癌旁组织 TP 蛋白表达与 MVD 正相关。 关键词：TP；肝细胞癌；免疫组织化学 II Abstr Abstract Abstract Objective: In this study, TP protein in liver tissue adjacent to carcinoma tissues, liver cancer tissues and portal venous tumor emboli are analyzed and MVD in liver tissue adjacent to carcinoma tissues were also detection. To investigate the relation of expression of TP and MVD in the tissue adjacent to hepatocellular carcinoma to explore the TPs potential value in the treatment of primary liver cell carcinoma metastasis. Methods: Specimens are taken from hepatocellular carcinoma patients, aged 30-76, the average age of 52, which were made a definite diagnosis by the affiliated Hospital of Hainan Medical College. In this study, TP proteins in liver tissue adjacent to carcinoma tissues, liver cancer tissues and portal venous tumor emboli were analyzed by immunohisto- chemical staining and the strength of the expression of TP were measured. MVD in the tissue adjacent to hepatocellular carcinoma were analyzed with anti CD34 markers and a statistical analysis was conducted in combination with clinic data. Results: 1、The expression of TP protein in the tissue adjacent to hepatocellular carcinoma and portal venous tumor emboli are significantly higher than liver cancer tissue. 2、The expression of TP protein in the tissue adja