不同剂量甲泼尼龙对大鼠急性百草枯中毒肾脏影响的研究-麻醉学专业论文.docxVIP

I I 摘 要 目的：1.探讨甲泼尼龙治疗大鼠百草枯中毒急性期肾脏损伤的疗效。 2.为激素在百草枯临床治疗中的应用及剂量选择提供参考。 方法：根据随机表法，将 120 只 Wistar 大鼠(体重在 200~250g)随机分为五组。空白组、染 毒对照组和甲泼尼龙干预组（根据甲泼尼龙剂量不同再分为三组：①5mg/kg.d，②15mg/kg.d， ③30mg/kg.d），除空白组外，其它各组均予百草枯（22mg/kg）稀释后腹腔注射，2h 后依照 组别、体重注射生理盐水稀释后的甲泼尼龙，在注射甲泼尼龙后的第 24h、72h、168h 共 3 个时间点，按抽签法每组各处死实验对象 6 只获取标本，观察一般情况、肾脏表面及剖面肉 眼变化、肾功能（血尿素氮及血肌酐）和病理变化。 结果：1.从组间因素看各组血尿素氮（P=0.0010.05）和血肌酐（P=0.010.05）差异有统 计学意义，干预组中 5mg/kg 甲泼尼龙组同染毒对照组比较血尿素氮和血肌酐显著改善。 2.从时间因素看不同时间点血尿素氮（P=0.0070.05）和血肌酐（P=0.0160.05）差 异有统计学意义，其中 168h 明显低于 24h、72h。 3.病理评分各组（P=0.210.05）差异无统计学意义。 结论：1.早期应用糖皮质激素治疗 PQ 中毒大鼠，可以显著减轻 PQ 中毒所致的肾损伤程度， 改善肾功能。 2.干预组中尤其以 5mg/kg 甲泼尼龙组改善显著，传统的大剂量糖皮质激素冲击治疗 不值的推崇。 关键词：百草枯，中毒，甲泼尼龙，血尿素氮，血肌酐，病理变化 论文类型：B（应用研究） II II Abstract Objection: 1.Studying effect of methylprednisolone treatment of renal injury in rats with paraquat poisoning. 2.It is a reference for the clinical application of GC on paraquat poisoning. Methods:According to the random table method, 120 Wistar rats （weight200~250g）were randomly divided into five groups, control group, exposed group and the intervention group (according to the different doses of methylprednisolone were divided into three groups: ①5mg/kg.d,②15mg/kg.d,③30mg/kg.d), intraperitoneal injection of paraquat(22mg/kg) except the control group, two hour later,according to group injected with different doses of methylprednisolone.24h,72h,168h in a total of three times, according to the experimental subjects were killed by drawing of lots 6 from every group to obtain samples of renal function and pathological changes. Results:1. Blood urea nitrogen (P=0.0010.05) and serum creatinine (P=0.010.05)in each group were significantly different；5mg methylprednisolone group was better than the others of the intervention group,which is significantly different with exposed group. Blood urea nitrogen (P=0.0070.05) and serum creatinine (P=0.0160.05) were significantly different at different time points, which was significantly lower than the72h and the 168h. Pathological