新加糖肾康对高糖环境下人肾小管上皮细胞α―平滑肌肌动蛋白和E―钙黏蛋白影响.docVIP

新加糖肾康对高糖环境下人肾小管上皮细胞α―平滑肌肌动蛋白和E―钙黏蛋白影响 　　摘要：目的 观察新加糖肾康对高糖环境培养下人肾小管上皮细胞（HK-2）α-平滑肌肌动蛋白（α-SMA）和E-钙黏蛋白（E-cadherin）的影响，探讨其防治糖尿病肾间质纤维化的作用机制。方法 含10%胎牛血清1640培养基体外培养HK-2细胞。实验分为空白对照组、高糖组、空白血清组和新加糖肾康低、中、高剂量组。药物干预后，MTT法检测细胞增殖，ELISA检测α-SMA和E-cadherin的含量。结果 与空白对照组比较，HK-2细胞经高糖培养后细胞数量和α-SMA含量显著增加、E-cadherin含量明显降低（P0.05）；与高糖组比较，新加糖肾康组α-SMA含量降低、E-cadherin含量增加、细胞增殖受到抑制。结论 新加糖肾康能够抑制肾小管上皮细胞表型转化及细胞增殖，具有防治糖尿病肾间质纤维化的作用。 　　关键词：新加糖肾康；高糖；人肾小管上皮细胞；表型转化；细胞增殖 　　DOI：10.3969/j.issn.1005-5304.2016.03.015 　　中图分类号：R285.5 文献标识码：A 文章编号：1005-5304（2016）03-0054-04 　　Abstract： Objective To observe he effects of new Tangshenkang on α-SMA and E-cadherin of human renal tubular epithelial cell HK-2 in high concentrations of glucose； To explore the mechanism of new Tangshenkang on the prevention and treatment of diabetic renal fibrosis. Methods The HK-2 cells were cultured and divided into control group， high glucose group， animal serum control group， new Tangshenkang low-， medium-， and high-dosage group. After medicine intervention， cell proliferation was tested by MTT assay， and contents of α-SMA and E-cadherin were observed by ELISA assay. Results Compared with control group， α-SMA of HK-2 cultured with high glucose was much notable， but the content of E-cadherin significantly decreased， with statistical significance （P0.05）. The content of α-SMA of HK-2 cultured with new Tangshenkang decreased， and the content of E-cadherin increased； cell proliferation was markedly inhibited in culture medium supernatant of HK-2 cells cultured with high glucose plus new Tangshenkang compared with only high glucose， with statistical significance. Conclusion New Tangshenkang can inhibit cell proliferation and epithelial-myofibroblast transdifferentiation of HK-2 cell induced by high glucose， and prevent the development of diabetic renal fibrosis to a certain extent. 　　Key words： new Tangshenkang； high glucose； human renal tubular epithelial cell HK-2； epithelial-myofibroblast transdifferentiation； cell proliferation