微波辅助法提取香菇多糖的工艺.pdfVIP

2010 年3 月 第31 卷第3 期 食食品研究与开发食品研究与开发食品研究与开发食品研究与开发食品研究与开发食品研究与开发食品研究与开发食品研究与开发食品研究与开发食品研究与开发食品研究与开发食品研究与开发食品研究与开发食品研究与开发食品研究与开发品研究与开发 基础研究 14 微波辅助法提取香菇多糖的工艺 刘小丽，黄晋杰 （长安大学环境科学与工程学院，陕西西安710054） 摘 要：研究微波辅助法提取香菇多糖并用离子交换树脂去除杂质蛋白的工艺方法。采用单因素试验对固液比、微 波辐射功率、辐射时间、乙醇用量以及杂蛋白的去除条件分别进行了考察。试验结果表明最佳提取工艺条件为: 固液 比1 g∶20 mL,微波辐射功率为280 W，辐射时间5 min，乙醇与多糖提取液体积比为4∶1，香菇多糖提取率可达到9.46 % ； 采用LX-67 阴离子交换树脂在料液pH 9 时可有效去除蛋白质杂质，多糖纯度可达到85 %。 关键词：香菇多糖；微波；离子交换树脂 Study on Microwave-assisted Extraction of Lentinan from Mushroom LIU Xiao-li ，HUANG Jin-jie （College of Environmental Science and Engineering ，Chang＇ an University ，Xian 710054, Shaanxi ，China） Abstract: The methods of extracting lentinan from mushroom with micowave assisting were studied. The ratio of solid to water （g/mL）, microwave power, microwave extraction time, the usage of ethanol, and the remove of protein were studied respectively by the single factor test. The results showed that the optimum conditions of extraction were as follows: the ratio of material to liquid of 1 g∶20 mL, microwave radiation power of 280 W, the radiation time for 5 min, the volume ratio of ethanol to liquor of 4 ∶1, under this conditions the extraction rate of polysaccharide can get 9.46 %. The LX-67 negative ion exchange resins were used to remove protein from the rude lentinan with the liquor at pH 9, and the purity of lentinan can get 85 %. Key words: lentinan；microwave ；ion exchange resins 香菇是我国传统的药食两用食品，含有多种有效 树脂, LX-200 型阴离子交换树脂，D201×7 型阴离子交 药用成分。尤其是香菇多糖,是一种宿主免疫增强剂, 换树脂，LX-67 型阴离子交换树脂：均购自西安蓝深公 具有抗病毒、抗肿瘤、调节免疫功能和刺激干扰素形 司; 葡萄糖, 苯酚,浓硫酸,无水乙醇,考马斯亮蓝G-205 成等功能。医学研究证明, 它是通过刺激机体的免疫 等均为分