猪OLR1基因多态性与肉质性状关联分析.docVIP

猪OLR1基因多态性与肉质性状关联分析 　　摘要：为鉴定与猪的肉质性状有重要影响的分子标记，经克隆测序，鉴定出OLR1基因第5内含子存在C52T突变，通过PCR-PstⅠ-RFLP方法进行了基因型检测，分别计算了该SNP在大白猪、长白猪、梅山猪、大白×梅山F2代猪中的基因型频率和基因频率，并且对大白×梅山F2代猪的基因型与肉质相关性状进行了关联分析。结果表明，CC基因型的肩部膘厚、胸腰椎间膘厚、平均背膘极显著高于CT基因型个体（P0.01）；CC基因型的皮率、瘦肉率、6-7腰椎间膘厚、花油重、臀部膘厚、背最长肌大理石纹、股二头肌大理石纹显著高于CT基因型（P0.05），C等位基因对猪的脂肪沉积具有正效应。 　　关键词：猪；OLR1；PCR-PstⅠ-RFLP；肉质性状；关联分析 　　中图分类号：S828 文献标识码：A 文章编号：0439-8114（2015）11-2686-04 　　DOI：10.14088/j.cnki.issn0439-8114.2015.11.034 　　Association Analysis on Meat Quality Traits and Polymorphism in Porcine OLR1 Gene 　　GUO Mi-mi1，MEI Shu-qi2，QIAO-Mu2，WU Jun-jing2，ZHAO Kang2，CHENG Yao1，LI Zhu-nan1 　　（1. College of Animal Science， Yangtze University， Jingzhou 434025， Hubei， China； 　　2.Institute of Animal Science and Veterinary Medicine， Hubei Academy of Agricultural Science， Wuhan 430064， China） 　　Abstract： To identify DNA markers which have significant effect on pig meat quality traits， the single nucleotide polymorphism （SNP） and genotype were tested by sequencing and PCR-PstⅠ-RFLP method，respectively. The results showed that a SNP （C52T） was found in intron 5，then the gene and genotype frequency in the Large White， Landrace， Meishan，Large White×Meishan F2’s populations and the association analysis was performed in Large White×Meishan F2’s pigs were analyzed. The results showed that the fat thickness， thorax-waist fat thickness and average backfat thickness of CC genotype pigs were extremely significantly higher than that of CT genotype（P0.01）； CC genotype pigs had significant higher skin percentage， lean meat percentage，6-7 rib fat thickness，dressing percentage， hip fat thickness， meat marbling of longissimus dorsi and meat marbling of biceps femoris than that of CT genotype（P0.05）. The results also showed that C allele had positive effect on fat deposition of pigs. 　　Key words： pig； OLR1； PCR-PstⅠ-RFLP； meat quality traits； association analysis 　　OLR1基因是动脉粥样硬化的候选基因，主要氧化低密度脂蛋白（OxLDL）内皮细胞，调节动脉粥样硬化病变和参与一些细胞过程，影响动脉粥样硬化的发病机制[1]。OLR1基因对血管内皮细胞的损伤、参与动脉粥样硬